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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1991-7-26
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pubmed:abstractText |
Macrophage-derived PGE2 is usually considered to be a down-regulator of TNF-alpha production. However, we recently demonstrated that PGE2 may display dual activities in that low concentrations stimulated whereas higher doses suppressed TNF-alpha synthesis in resident peritoneal macrophages. To examine the underlying molecular mechanisms, we studied TNF-alpha gene expression in rat peritoneal macrophages and the murine PU5-1.8 macrophage line. In both macrophage types, PGE2 enhanced TNF-alpha gene transcription and production at an optimal concentration of 1 ng/ml. Furthermore, evidence was obtained that PGE2 may stimulate TNF-alpha mRNA accumulation via a rise of the intracellular messenger cGMP. Both, exogenously added as well as endogenously, by sodium nitroprusside generated cGMP were found to enhance TNF-alpha gene expression and production. These findings lend further support to the concept that cGMP may represent one of the positive signals for TNF-alpha synthesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0171-2985
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
182
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
44-55
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1965895-Animals,
pubmed-meshheading:1965895-Blotting, Northern,
pubmed-meshheading:1965895-Cell Line,
pubmed-meshheading:1965895-Cells, Cultured,
pubmed-meshheading:1965895-Cyclic GMP,
pubmed-meshheading:1965895-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:1965895-Dinoprostone,
pubmed-meshheading:1965895-Female,
pubmed-meshheading:1965895-Gene Expression Regulation,
pubmed-meshheading:1965895-Kinetics,
pubmed-meshheading:1965895-Macrophages,
pubmed-meshheading:1965895-Male,
pubmed-meshheading:1965895-Mice,
pubmed-meshheading:1965895-Nitroprusside,
pubmed-meshheading:1965895-Peritoneal Cavity,
pubmed-meshheading:1965895-RNA, Messenger,
pubmed-meshheading:1965895-Rats,
pubmed-meshheading:1965895-Rats, Inbred Lew,
pubmed-meshheading:1965895-Second Messenger Systems,
pubmed-meshheading:1965895-Transcription, Genetic,
pubmed-meshheading:1965895-Tumor Necrosis Factor-alpha,
pubmed-meshheading:1965895-Up-Regulation
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pubmed:year |
1990
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pubmed:articleTitle |
Enhancement of tumor necrosis factor-alpha gene expression by low doses of prostaglandin E2 and cyclic GMP.
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pubmed:affiliation |
Institute of Immunology, Philipps University, Marburg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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