Linked Life Data

19647072

Source:http://linkedlifedata.com/resource/pubmed/id/19647072

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2009-9-23
pubmed:abstractText
Chaga (Inonotus obliquus (persoon) Pilat) is a mushroom traditionally used as a folk medicine for tumors and stomach ulcers in Russia. Previously, we reported the antioxidant potential of Chaga extracts and seven isolated phenolic ingredients. In the present study, we investigated the protective effects of Chaga extracts and other isolated phenolic ingredients against H(2)O(2)-induced oxidative stress in PC12 cells. Intracellular generation of reactive oxygen species (ROS) leads to oxidative stress and subsequent damage of cellular and nuclear components. Chaga extracts and the phenolic ingredients, 3,4-dihydroxybenzalacetone (DBL) and caffeic acid (CA), effectively suppressed intracellular ROS level in H(2)O(2)-treated cells. The H(2)O(2)-induced cell death was more pronounced, effectively prevented in the cells treated with DBL than in cells treated with CA. In addition, ROS activate various signal transduction pathways including the mitogen-activated protein kinase (MAPK) cascade. Therefore, we examined the potentially beneficial effects of DBL on extracellular signal-regulated protein kinase (ERK), c-Jun NH(2)-terminal kinase (JNK), and p38-MAPK signaling activated by H(2)O(2) stimulation. DBL selectively inhibited the phosphorylation of p38-MAPK, without affecting JNK and ERK.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1873-4596
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1154-61
pubmed:meshHeading
pubmed-meshheading:19647072-Animals, pubmed-meshheading:19647072-Apoptosis, pubmed-meshheading:19647072-Basidiomycota, pubmed-meshheading:19647072-Blotting, Western, pubmed-meshheading:19647072-Caffeic Acids, pubmed-meshheading:19647072-Hydrogen Peroxide, pubmed-meshheading:19647072-JNK Mitogen-Activated Protein Kinases, pubmed-meshheading:19647072-Membrane Potential, Mitochondrial, pubmed-meshheading:19647072-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:19647072-Mitogen-Activated Protein Kinase 3, pubmed-meshheading:19647072-Oxidants, pubmed-meshheading:19647072-Oxidative Stress, pubmed-meshheading:19647072-PC12 Cells, pubmed-meshheading:19647072-Phosphorylation, pubmed-meshheading:19647072-Rats, pubmed-meshheading:19647072-Reactive Oxygen Species, pubmed-meshheading:19647072-p38 Mitogen-Activated Protein Kinases
pubmed:year
2009
pubmed:articleTitle
Prevention of hydrogen peroxide-induced oxidative stress in PC12 cells by 3,4-dihydroxybenzalacetone isolated from Chaga (Inonotus obliquus (persoon) Pilat).
pubmed:affiliation
Niigata University of Pharmacy and Applied Life Science, Higashijima, Akiha-ku, Niigata 956-8603, Japan.
pubmed:publicationType
Journal Article