Linked Life Data

19646425

Source:http://linkedlifedata.com/resource/pubmed/id/19646425

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-9-18
pubmed:abstractText
Cyclic adenosine monophosphate (cAMP) has been intensively studied in recent years in order to elucidate its contribution in intracellular signalling mechanisms that regulate axon growth and guidance, and also to test if its activation can promote axon regeneration after injury. Cyclic guanosine monophosphate (cGMP), however, has been given considerably less attention even though it too mediates intracellular signalling cascades activated by extracellular guidance cues. cGMP can promote neurite outgrowth in neuronal cell lines but its role in promoting growth and regeneration of primary neurons is not well established. Here, we have examined the effects of elevating cGMP activity on axon growth, guidance and regeneration in vitro. We have found that, like cAMP elevation, activation of cGMP increases rat dorsal root ganglion (DRG) neurite outgrowth on a polylysine substrate and that asymmetric cGMP elevation promotes attractive growth cone turning. When grown in an in vitro model of axon regeneration activation of cGMP alone was not sufficient to promote adult neurite outgrowth. However, when combined with cAMP elevation substantial regeneration of adult neurites is achieved, superior to that achieved with either cAMP or cGMP alone. Regeneration is enhanced still further with simultaneous application of a Nogo receptor blocking peptide, suggesting this combinatorial strategy could achieve far greater axon regeneration in vivo than targeting individual cell signalling mechanisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1872-6240
pubmed:author
pubmed:issnType
Electronic
pubmed:day
19
pubmed:volume
1294
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12-21
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:19646425-Aging, pubmed-meshheading:19646425-Animals, pubmed-meshheading:19646425-Animals, Newborn, pubmed-meshheading:19646425-Axons, pubmed-meshheading:19646425-Cells, Cultured, pubmed-meshheading:19646425-Cyclic AMP, pubmed-meshheading:19646425-Cyclic AMP Response Element-Binding Protein, pubmed-meshheading:19646425-Cyclic GMP, pubmed-meshheading:19646425-GPI-Linked Proteins, pubmed-meshheading:19646425-Ganglia, Spinal, pubmed-meshheading:19646425-Growth Cones, pubmed-meshheading:19646425-Immunohistochemistry, pubmed-meshheading:19646425-Myelin Proteins, pubmed-meshheading:19646425-Nerve Regeneration, pubmed-meshheading:19646425-Neurites, pubmed-meshheading:19646425-Neurons, pubmed-meshheading:19646425-Phosphorylation, pubmed-meshheading:19646425-Rats, pubmed-meshheading:19646425-Rats, Sprague-Dawley, pubmed-meshheading:19646425-Receptors, Cell Surface, pubmed-meshheading:19646425-Receptors, Peptide, pubmed-meshheading:19646425-Spinal Cord
pubmed:year
2009
pubmed:articleTitle
cGMP promotes neurite outgrowth and growth cone turning and improves axon regeneration on spinal cord tissue in combination with cAMP.
pubmed:affiliation
School of Medical Sciences, College of Life Sciences and Medicine, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK. a.j.murray@abdn.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't