Linked Life Data

19625773

Source:http://linkedlifedata.com/resource/pubmed/id/19625773

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-9-8
pubmed:abstractText
The development of denticle rows on the ventral Drosophila embryo is a valuable system for studying the genetic control of epithelial patterning. During late embryogenesis, the apical surfaces of denticle-producing cells acquire a distinctive rectangular morphology with long anteroposterior boundaries, along which the denticles form, and short ventrolateral boundaries that stain strongly for adherens junction proteins. We observe that ventrolateral denticle cell boundaries are also convoluted, suggesting that the strong adherens staining results, at least in part, from the additional membrane in these regions. Embryos mutant for the Planar Cell Polarity (PCP) Effector gene multiple wing hairs (mwh), or expressing dominant negative form of the small GTPase Rac1, have cells present between the normal denticle cell rows. These 'Interloper Cells' do not have convoluted ventrolateral boundaries with strong adherens protein staining, but have normal denticle placement, suggesting that adherens protein localization is not critical for denticle cell PCP. Based on these and other observations, we propose that denticle cell morphology arises from an epithelial stretch without junction remodeling. A crude mechanical model suggests that this mechanism can generate both the straight anteroposterior boundaries and the compacted ventrolateral boundaries typical of denticle cells. We discuss the significance of cell adhesion for denticle cell morphogenesis, especially given the established role for Rac1 in cell adhesion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1933-6942
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
185-91
pubmed:meshHeading
pubmed:articleTitle
Cell shape and epithelial patterning in the Drosophila embryonic epidermis.
pubmed:affiliation
Department of Biological Sciences, Marshall University, Huntington, WV, USA.
pubmed:publicationType
Journal Article