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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2009-7-7
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pubmed:abstractText |
To determine whether increased mitochondrially localized catalase was radioprotective, a human catalase transgene was cloned into a small pSVZeo plasmid and localized to the mitochondria of 32D cl 3 cells by adding the mitochondrial localization sequence of MnSOD (mt-catalase). The cell lines 32D-Cat and 32D-mt-Cat had increased catalase biochemical activity as confirmed by Western blot analysis compared to the 32D cl 3 parent cells. The MnSOD-overexpressing 32D cl 3 cell line, 2C6, had decreased baseline catalase activity that was increased in 2C6-Cat and 2C6-mt-Cat subclonal cell lines. 32D-mt-Cat cells were more radioresistant than 32D-Cat cells, but both were radioresistant relative to 32D cl 3 cells. 2C6-mt-Cat cells but not 2C6-Cat cells were radioresistant compared to 2C6 cells. Intratracheal injection of the mt-catalase-plasmid liposome complex (mt-Cat-PL) but not the catalase-plasmid liposome complex (Cat-PL) increased the resistance of C57BL/6NHsd female mice to 20 Gy thoracic irradiation compared to MnSOD-plasmid liposomes. Thus mitochondrially targeted overexpression of the catalase transgene is radioprotective in vitro and in vivo.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-01,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-010001,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-015637,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-019006,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-019007,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-02,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-020001,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-029006,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-029007,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-03,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-030001,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-039006,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-039007,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-04,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-040001,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-049006,
http://linkedlifedata.com/resource/pubmed/grant/U19 AI068021-049007,
http://linkedlifedata.com/resource/pubmed/grant/U19-A1068021
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-10473575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-10802227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-10863528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-11121210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-11873514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-11891202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-12571645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-12600239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-12762478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-14565825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-14575837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-14633599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-14657354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-14967442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-15197331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-15466388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-15520863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-15701646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-15750616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-16278309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-17316075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-17595331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-17700625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580494-7574505
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0033-7587
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pubmed:author |
pubmed-author:DixonTracyT,
pubmed-author:EpperlyMichael WMW,
pubmed-author:FranicolaDarcyD,
pubmed-author:GreenbergerBenjamin ABA,
pubmed-author:GreenbergerJoel SJS,
pubmed-author:KomanduriPaavaniP,
pubmed-author:MelendezJ AJA,
pubmed-author:NieSuhuaS,
pubmed-author:PearceLindaL,
pubmed-author:PetersonJamesJ,
pubmed-author:WangHongH,
pubmed-author:ZhangXichenX
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pubmed:issnType |
Print
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pubmed:volume |
171
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
588-95
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pubmed:dateRevised |
2011-5-10
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pubmed:meshHeading |
pubmed-meshheading:19580494-Humans,
pubmed-meshheading:19580494-Animals,
pubmed-meshheading:19580494-Mice,
pubmed-meshheading:19580494-Catalase,
pubmed-meshheading:19580494-Glutathione,
pubmed-meshheading:19580494-Female,
pubmed-meshheading:19580494-Mitochondria,
pubmed-meshheading:19580494-Cell Survival,
pubmed-meshheading:19580494-Cell Line,
pubmed-meshheading:19580494-Radiation Tolerance,
pubmed-meshheading:19580494-Mice, Inbred C57BL,
pubmed-meshheading:19580494-Plasmids,
pubmed-meshheading:19580494-Liposomes,
pubmed-meshheading:19580494-Superoxide Dismutase,
pubmed-meshheading:19580494-Glutathione Peroxidase
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