Linked Life Data

19523887

Source:http://linkedlifedata.com/resource/pubmed/id/19523887

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2009-6-29
pubmed:abstractText
The method for refolding of mini-antibodies using size-exclusion chromatography via arginine solution layer was developed. This method allows to refold scFv, to separate both aggregated protein and low molecular weight compounds and to isolate functionally active protein preparation in monomeric form. The comparison of various scFv preparations isolated either from inclusion bodies or from soluble fraction revealed that refolded mini-antibodies demonstrate higher antigen-binding activity. Mini-antibodies refolded in the presence of arginine also demonstrate higher electrophoretic mobility during native PAGE in comparison with soluble cytoplasmic antibodies. Both soluble as well as refolded antibodies had similar CD spectra. Refolded mini-antibodies are storage-stable.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1873-376X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
877
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2045-51
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Refolding of scFv mini-antibodies using size-exclusion chromatography via arginine solution layer.
pubmed:affiliation
Branch Shemyakin&Ovchinikov Institute Bioorganic Chemistry RAS, Science Avenue b.6, 142290, Pushchino, Moscow Region, Russian Federation.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies