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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2009-7-15
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pubmed:abstractText |
Melatonin possesses anti-estrogenic effects on estrogen receptor expressing (ER+) breast cancer cells in culture by reducing cell cycle progression and cell proliferation. There is increasing agreement that on a cellular level the effects of melatonin are primarily induced by the membrane-bound receptor MT1. The participation of a second, nuclear receptor of the group of ligand-dependent transcription factors, called RZRalpha, is under debate. In this study we used a number of breast cancer cell lines differing in their expression of the estrogen receptor and the two known melatonin receptors. In MCF-7 breast cancer cells transfected with a vector carrying the MT1 gene (MCF-7Mel1a) binding of CREB-protein to the cAMP-responsive element of the breast cancer suppressing gene BRCA-1 was more strongly reduced by treatment with melatonin than in the parental cells. Expression of estrogen responsive genes was determined in serum-starved cells, cells stimulated for 16 hr with estradiol and cells subsequently treated with melatonin. Expression of BRCA-1, p53, p21(WAF) and c-myc were up-regulated by estradiol. Treatment of the stimulated cells with melatonin counteracted the increase induced by estradiol almost completely. The more MT1 a cell line expressed, the stronger was the reduction of the expression of the estradiol-induced genes. There was no correlation between the expression of the nuclear receptor RZRalpha and the effects of melatonin on these genes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/BRCA1 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/BRCA1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CDKN1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/MYC protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Melatonin,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myc,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Melatonin, MT1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Estrogen,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1600-079X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23-31
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pubmed:meshHeading |
pubmed-meshheading:19522736-BRCA1 Protein,
pubmed-meshheading:19522736-Breast Neoplasms,
pubmed-meshheading:19522736-Cell Cycle,
pubmed-meshheading:19522736-Cell Growth Processes,
pubmed-meshheading:19522736-Cell Line, Tumor,
pubmed-meshheading:19522736-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:19522736-Down-Regulation,
pubmed-meshheading:19522736-Estradiol,
pubmed-meshheading:19522736-Female,
pubmed-meshheading:19522736-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:19522736-Humans,
pubmed-meshheading:19522736-Melatonin,
pubmed-meshheading:19522736-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:19522736-Receptor, Melatonin, MT1,
pubmed-meshheading:19522736-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:19522736-Receptors, Estrogen,
pubmed-meshheading:19522736-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19522736-Tumor Suppressor Protein p53
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pubmed:year |
2009
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pubmed:articleTitle |
Membrane-bound melatonin receptor MT1 down-regulates estrogen responsive genes in breast cancer cells.
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pubmed:affiliation |
Department of Obstetrics and Gynecology, University of Göttingen, Göttingen, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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