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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2009-6-18
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pubmed:abstractText |
Macrophage colony-stimulating factor (M-CSF) influences the proliferation and survival of mononuclear phagocytes through the receptor CSF-1R. The adaptor protein DAP12 is critical for the function of mononuclear phagocytes. DAP12-mutant mice and humans have defects in osteoclasts and microglia, as well as brain and bone abnormalities. Here we show DAP12 deficiency impaired the M-CSF-induced proliferation and survival of macrophages in vitro. DAP12-deficient mice had fewer microglia in defined central nervous system areas, and DAP12-deficient progenitors regenerated myeloid cells inefficiently after bone marrow transplantation. Signaling by M-CSF through CSF-1R induced the stabilization and nuclear translocation of beta-catenin, which activated genes involved in the cell cycle. DAP12 was essential for phosphorylation and nuclear accumulation of beta-catenin. Our results provide a mechanistic explanation for the many defects of DAP12-deficient mononuclear phagocytes.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Aif1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Colony-Stimulating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ptk2b protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrobp protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1529-2916
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pubmed:author |
pubmed-author:AoshiTaikiT,
pubmed-author:CeruttiElisaE,
pubmed-author:ColonnaMarcoM,
pubmed-author:MillerMarkM,
pubmed-author:OteroKarelK,
pubmed-author:PolianiPietro LuigiPL,
pubmed-author:ShawAndrey SAS,
pubmed-author:StanleySamuel LSL,
pubmed-author:TakaiToshiyukiT,
pubmed-author:TassiIlariaI,
pubmed-author:TurnbullIsaiah RIR,
pubmed-author:VermiWilliamW
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pubmed:issnType |
Electronic
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
734-43
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:19503107-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:19503107-Animals,
pubmed-meshheading:19503107-Brain,
pubmed-meshheading:19503107-Calcium-Binding Proteins,
pubmed-meshheading:19503107-Cell Adhesion,
pubmed-meshheading:19503107-Cell Cycle,
pubmed-meshheading:19503107-Cell Proliferation,
pubmed-meshheading:19503107-Cell Survival,
pubmed-meshheading:19503107-Cells, Cultured,
pubmed-meshheading:19503107-Flow Cytometry,
pubmed-meshheading:19503107-Focal Adhesion Kinase 2,
pubmed-meshheading:19503107-Green Fluorescent Proteins,
pubmed-meshheading:19503107-Immunoblotting,
pubmed-meshheading:19503107-Immunohistochemistry,
pubmed-meshheading:19503107-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:19503107-Macrophages,
pubmed-meshheading:19503107-Mice,
pubmed-meshheading:19503107-Mice, Inbred C57BL,
pubmed-meshheading:19503107-Mice, Inbred Strains,
pubmed-meshheading:19503107-Mice, Knockout,
pubmed-meshheading:19503107-Mice, Transgenic,
pubmed-meshheading:19503107-Microfilament Proteins,
pubmed-meshheading:19503107-Phosphorylation,
pubmed-meshheading:19503107-Signal Transduction,
pubmed-meshheading:19503107-beta Catenin
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pubmed:year |
2009
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pubmed:articleTitle |
Macrophage colony-stimulating factor induces the proliferation and survival of macrophages via a pathway involving DAP12 and beta-catenin.
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pubmed:affiliation |
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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