Linked Life Data

19500473

Source:http://linkedlifedata.com/resource/pubmed/id/19500473

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2009-12-2
pubmed:abstractText
We aimed to evaluate the feasibility and efficacy of autologous umbilical cord blood mononuclear cell (UCMNC) transplantation on right ventricular (RV) function in a novel model of chronic RV volume overload. Four-month-old sheep (n = 20) were randomized into cell (n = 10) and control groups (n = 10). After assessment of baseline RV function by the conductance catheter method, a transannular patch (TAP) was sutured to the right ventricular outflow tract (RVOT). Following infundibulotomy the ring of the pulmonary valve was transected without cardiopulmonary bypass. UCMNC implantation (8.22 +/- 6.28 x 10(7)) in the cell group and medium injection in the control group were performed into the RV myocardium around the TAP. UCMNCs were cultured for 2 weeks after fluorescence-activated cell sorting (FACS) analysis for CD34 antigen. Transthoracic echocardiography (TTE) and computed tomography were performed after 6 weeks and 3 months, respectively. RV function was assessed 3 months postoperatively before the hearts were excised for immunohistological examinations. FACS analysis revealed 1.2 +/- 0.22% CD34(+) cells within the isolated UCMNCs from which AcLDL(+) endothelial cells were cultured in vitro. All animals survived surgery. TTE revealed grade II-III pulmonary regurgitation in both groups. Pressure-volume loops under dobutamine stress showed significantly improved RV diastolic function in the cell group (dP/dt(min): p = 0.043; E(ed): p = 0.009). CD31 staining indicated a significantly enhanced number of microvessels in the region of UCMNC implantation in the cell group (p < 0.001). No adverse tissue changes were observed. TAP augmentation and pulmonary annulus distortion without cardiopulmonary bypass constitutes a valid large animal model mimicking the surgical repair of tetralogy of Fallot. Our results indicate that the chronically volume-overloaded RV profits from autologous UCMNC implantation by enhanced diastolic properties with a probable underlying mechanism of increased angiogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1555-3892
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
855-68
pubmed:meshHeading
pubmed-meshheading:19500473-Animals, pubmed-meshheading:19500473-Cardiac Surgical Procedures, pubmed-meshheading:19500473-Cardiac Volume, pubmed-meshheading:19500473-Cells, Cultured, pubmed-meshheading:19500473-Chronic Disease, pubmed-meshheading:19500473-Cord Blood Stem Cell Transplantation, pubmed-meshheading:19500473-Echocardiography, pubmed-meshheading:19500473-Hypertrophy, Right Ventricular, pubmed-meshheading:19500473-Leukocytes, Mononuclear, pubmed-meshheading:19500473-Postoperative Complications, pubmed-meshheading:19500473-Random Allocation, pubmed-meshheading:19500473-Sheep, pubmed-meshheading:19500473-Transplantation, Autologous, pubmed-meshheading:19500473-Ventricular Dysfunction, Right, pubmed-meshheading:19500473-Ventricular Function, Right, pubmed-meshheading:19500473-Ventricular Outflow Obstruction
pubmed:year
2009
pubmed:articleTitle
Autologous umbilical cord blood mononuclear cell transplantation preserves right ventricular function in a novel model of chronic right ventricular volume overload.
pubmed:affiliation
Department of Cardiac Surgery, Medical Faculty, University of Rostock, Rostock, Germany. can.yerebakan@med.uni-rostock.de
pubmed:publicationType
Journal Article, Evaluation Studies