Linked Life Data

19432670

Source:http://linkedlifedata.com/resource/pubmed/id/19432670

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2009-5-12
pubmed:abstractText
Microwave cell death (MCD) is unique in that microscopic morphology and antigenicity are well preserved, while enzymatic activity is lost. The aim of the present study was to carry out DNA electrophoresis, polymerase chain reaction (PCR) amplification and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) examination of MCD. DNA electrophoresis indicated only a thick band of large DNA immediately after microwave treatment, which became a smear 6 h later and indicated a delayed type of DNA degradation over 2 weeks. Neither a DNA smear characteristic of oncosis nor DNA ladder patterns characteristic of apoptosis were found in MCD. PCR of p53 in MCD showed a single band at the same level of control of non-microwave treated cells. MCD was negatively stained on TUNEL. These results provide further evidence that MCD is distinct from oncosis and apoptosis, in addition to showing unique morphological and antigenic preservation and enzymatic inactivation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1440-1827
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
294-9
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Microwave cell death: molecular analysis using DNA electrophoresis, PCR amplification and TUNEL.
pubmed:affiliation
Department of Pathology, Wakayama Medical University, Wakayama City, Wakayama, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't