Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2009-10-27
pubmed:abstractText
We found that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil delivery can be used to label and purify cell type-specific RNA from intact complex tissues in Drosophila melanogaster. This method is useful for isolating RNA from cell types that are difficult to isolate by dissection or dissociation methods and should work in many organisms, including mammals and other vertebrates.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19430475-12214599, http://linkedlifedata.com/resource/pubmed/commentcorrection/19430475-12653656, http://linkedlifedata.com/resource/pubmed/commentcorrection/19430475-15685165, http://linkedlifedata.com/resource/pubmed/commentcorrection/19430475-16213219, http://linkedlifedata.com/resource/pubmed/commentcorrection/19430475-16772168, http://linkedlifedata.com/resource/pubmed/commentcorrection/19430475-16962313, http://linkedlifedata.com/resource/pubmed/commentcorrection/19430475-17645804, http://linkedlifedata.com/resource/pubmed/commentcorrection/19430475-18245445, http://linkedlifedata.com/resource/pubmed/commentcorrection/19430475-18369980, http://linkedlifedata.com/resource/pubmed/commentcorrection/19430475-18658122, http://linkedlifedata.com/resource/pubmed/commentcorrection/19430475-19013281, http://linkedlifedata.com/resource/pubmed/commentcorrection/19430475-19013282, http://linkedlifedata.com/resource/pubmed/commentcorrection/19430475-19015660, http://linkedlifedata.com/resource/pubmed/commentcorrection/19430475-7763479, http://linkedlifedata.com/resource/pubmed/commentcorrection/19430475-7916657
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1548-7105
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
439-41
pubmed:dateRevised
2011-2-21
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
TU-tagging: cell type-specific RNA isolation from intact complex tissues.
pubmed:affiliation
Institute of Neuroscience, Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural