Linked Life Data

19361925

Source:http://linkedlifedata.com/resource/pubmed/id/19361925

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-5-13
pubmed:abstractText
Although N-methyl-d-aspartate (NMDA) receptor antagonists potentiate antinociceptive effects induced by various exogenous opioids at the spinal, supraspinal, or peripheral level, less is known regarding the interaction between NMDA and endogenous opioids in antinociception. We therefore assessed the effects of NMDA receptor antagonists on endogenous opioids in antinociception at the peripheral level by testing the ability of the locally administered receptor antagonists to modify pain-related behavior induced by carrageenan injection into the knee joint. The NMDA receptor antagonist AP-5 or the exogenous opioid morphine was injected intra-articularly before carrageenan injection and 5h after carrageenan injection, respectively. We evaluated whether intra-articular injection of the opioid receptor antagonist naloxone reversed the analgesic effect of AP-5. In addition, we tested the effects of AP-5 on carrageenan-induced levels of the beta-endorphin protein in dorsal root ganglia (DRG), saphenous nerve and synovial membrane. We found that AP-5 prevented and morphine reversed carrageenan-induced pain-related behavior. Intriguingly, injection of naloxone 5h after carrageenan injection reversed the antinociceptive effects of AP-5 pre-treatment, although naloxone alone had no effect on carrageenan-induced pain-related behavior. Western blots showed that AP-5 pre-treatment followed by carrageenan injection resulted in a higher level of beta-endorphin protein in the DRG and saphenous nerve, but not in the synovial membrane, than that observed following saline control treatment. These results suggest that inhibition of the NMDA receptor unmasks antinociception induced by endogenous opioids at the peripheral level, partly through the increased protein level of the endogenous mu-opioid peptide beta-endorphin in DRG and saphenous nerve.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1872-6623
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
143
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
233-7
pubmed:meshHeading
pubmed-meshheading:19361925-2-Amino-5-phosphonovalerate, pubmed-meshheading:19361925-Analgesics, Opioid, pubmed-meshheading:19361925-Animals, pubmed-meshheading:19361925-Arthralgia, pubmed-meshheading:19361925-Arthritis, pubmed-meshheading:19361925-Carrageenan, pubmed-meshheading:19361925-Drug Synergism, pubmed-meshheading:19361925-Excitatory Amino Acid Antagonists, pubmed-meshheading:19361925-Ganglia, Spinal, pubmed-meshheading:19361925-Male, pubmed-meshheading:19361925-Morphine, pubmed-meshheading:19361925-Naloxone, pubmed-meshheading:19361925-Narcotic Antagonists, pubmed-meshheading:19361925-Opioid Peptides, pubmed-meshheading:19361925-Pain, pubmed-meshheading:19361925-Pain Measurement, pubmed-meshheading:19361925-Pain Threshold, pubmed-meshheading:19361925-Peripheral Nerves, pubmed-meshheading:19361925-Rats, pubmed-meshheading:19361925-Rats, Sprague-Dawley, pubmed-meshheading:19361925-Receptors, N-Methyl-D-Aspartate, pubmed-meshheading:19361925-Synovial Membrane, pubmed-meshheading:19361925-beta-Endorphin
pubmed:year
2009
pubmed:articleTitle
Inhibition of the N-methyl-d-aspartate receptor unmasks the antinociception of endogenous opioids in the periphery.
pubmed:affiliation
Department of Physiology, College of Medicine, Korea University, 126-1 Anam-dong 5 ga, Sungbuk-gu, Seoul 136-705, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't