Source:http://linkedlifedata.com/resource/pubmed/id/19350081
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2009-4-7
|
| pubmed:abstractText |
We report the utilization of microfluidic technology to phage selection and demonstrate that accurate control of washing stringency in our microfluidic magnetic separator (MMS) directly impacts the diversity of isolated peptide sequences. Reproducible generation of magnetic and fluidic forces allows controlled washing conditions that enable rapid convergence of selected peptide sequences. These findings may provide a foundation for the development of automated microsystems for rapid in vitro directed evolution of affinity reagents.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
1473-0197
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
21
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1033-6
|
| pubmed:meshHeading |
pubmed-meshheading:19350081-Amino Acid Sequence,
pubmed-meshheading:19350081-Magnetics,
pubmed-meshheading:19350081-Microfluidic Analytical Techniques,
pubmed-meshheading:19350081-Molecular Sequence Data,
pubmed-meshheading:19350081-Peptide Library,
pubmed-meshheading:19350081-Peptides,
pubmed-meshheading:19350081-Quality Control,
pubmed-meshheading:19350081-Reproducibility of Results,
pubmed-meshheading:19350081-Sensitivity and Specificity
|
| pubmed:year |
2009
|
| pubmed:articleTitle |
Controlling the selection stringency of phage display using a microfluidic device.
|
| pubmed:affiliation |
Neuroscience Research Institute, University of California, Santa Barbara, CA93106, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|