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rdf:type |
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lifeskim:mentions |
umls-concept:C0011306,
umls-concept:C0021311,
umls-concept:C0086538,
umls-concept:C0109317,
umls-concept:C0127400,
umls-concept:C0205349,
umls-concept:C0752312,
umls-concept:C0871261,
umls-concept:C1150579,
umls-concept:C1318444,
umls-concept:C1333340,
umls-concept:C1366882,
umls-concept:C1370600,
umls-concept:C1704632,
umls-concept:C1705767,
umls-concept:C1705791,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
5
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pubmed:dateCreated |
2009-4-27
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pubmed:abstractText |
Initiation of productive immune responses against Leishmania depends on the successful transition of dendritic cells (DC) from an immature to a mature phenotype. This process is characterized by high CD40 surface expression as well as interleukin-12 production, which are frequently seen in response to L. major infection. In vivo footpad infection of C3HeB/FeJ mice for 7 days with L. amazonensis promoted an immature CD11c(+) DC phenotype characterized by both significantly low CD40 surface expression and significantly decreased interleukin-12p40 production compared with L. major infection of these same mice. In vitro infection of bone marrow-derived dendritic cells with L. amazonensis amastigotes resulted in rapid and significant phosphorylation of the mitogen activated protein kinase, extracellular signal-regulated kinase 1/2, observed within minutes of exposure to the parasite. Infection with L. amazonensis promastigotes led to increased 1/2 phosphorylation after 4 hours of infection compared with L. major infection, which correlated with promastigote transformation into amastigotes. Treatment of bone marrow-derived dendritic cells with a mitogen activated protein kinase kinase-specific inhibitor, PD98059, led to regained surface CD40 expression and interleukin-12p40 production following L. amazonensis amastigote infection compared with non-treated, infected DC. Treatment of L. amazonensis-infected mice with the highly-specific mitogen activated protein kinase kinase inhibitor, CI-1040, enhanced surface CD40 expression on CD11c(+) DC obtained from the draining lymph node. L. amazonensis amastigotes, through activation of extracellular signal-regulated kinase 1/2, inhibit the ability of DC to undergo proper maturation both in vitro and in vivo.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-10037236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-10586030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-10861073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-11242034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-11895981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-12164286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-12706437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-15573115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-15831826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-16212907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-16267021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-16352421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-16495525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-16818784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-17543969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-17682041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-18538399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-7612219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-8624816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-8624817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-8624818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-8766560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-9509670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-9521319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19349356-9841930
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1525-2191
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
174
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1818-26
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pubmed:dateRevised |
2010-9-24
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pubmed:meshHeading |
pubmed-meshheading:19349356-Animals,
pubmed-meshheading:19349356-Antigens, CD11c,
pubmed-meshheading:19349356-Antigens, CD40,
pubmed-meshheading:19349356-Bone Marrow,
pubmed-meshheading:19349356-Dendritic Cells,
pubmed-meshheading:19349356-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:19349356-Female,
pubmed-meshheading:19349356-Flow Cytometry,
pubmed-meshheading:19349356-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:19349356-Host-Parasite Interactions,
pubmed-meshheading:19349356-Immunoblotting,
pubmed-meshheading:19349356-Leishmania,
pubmed-meshheading:19349356-Mice,
pubmed-meshheading:19349356-Mice, Inbred C3H,
pubmed-meshheading:19349356-Mice, SCID,
pubmed-meshheading:19349356-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:19349356-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:19349356-Mitogen-Activated Protein Kinases,
pubmed-meshheading:19349356-Phenotype,
pubmed-meshheading:19349356-Phosphorylation
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pubmed:year |
2009
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pubmed:articleTitle |
Altered dendritic cell phenotype in response to Leishmania amazonensis amastigote infection is mediated by MAP kinase, ERK.
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pubmed:affiliation |
Department of Veterinary Pathology, Iowa State University, Ames, Iowa 50011, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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