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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1991-12-3
|
| pubmed:abstractText |
We demonstrate that PCR amplification of human genomic DNA can be used for the detection of loss of heterozygosity (LOH) in tumor samples. A 250-bp fragment containing codon 72 of the human p53 gene was amplified, ThaI digested and electrophoresed. Tumor LOH is detectable both by ethidium bromide staining and autoradiography, despite 25% contamination with normal DNA. This technique provides a fast and reproducible alternative to conventional Southern blotting and has minimal sample requirements.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0736-6205
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
11
|
| pubmed:geneSymbol |
TP53
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
166-8, 170-1
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:1931011-Base Sequence,
pubmed-meshheading:1931011-Blotting, Southern,
pubmed-meshheading:1931011-Breast Neoplasms,
pubmed-meshheading:1931011-DNA, Neoplasm,
pubmed-meshheading:1931011-Heterozygote Detection,
pubmed-meshheading:1931011-Humans,
pubmed-meshheading:1931011-Molecular Sequence Data,
pubmed-meshheading:1931011-Polymerase Chain Reaction
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Detection of loss of heterozygosity in tumor DNA samples by PCR.
|
| pubmed:affiliation |
Oncogenetic Section, NCI, NIH, Bethesda, MD 20892.
|
| pubmed:publicationType |
Journal Article
|