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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7-8
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pubmed:dateCreated |
2009-6-1
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pubmed:abstractText |
Cellular and molecular events in vascular smooth muscle cells (VSMC) from Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were investigated. SHR-derived VSMC showed increased proliferative capacity and MAP kinase levels in comparison with WKY-derived VSMC. Flow cytometry analysis revealed that progression from G1 to S phase was faster in SHR-derived VSMC in response to tumor necrosis factor-alpha (TNF-alpha) as compared with cells from WKY. The G1 cell cycle-associated proteins such as cyclin D1, cyclin E, CDK2 and CDK4, and kinase activities associated with CDK2 and CDK4, were increased in SHR-derived VSMC. In addition, CDK inhibitor p21 was elevated in SHR-derived cells. Matrix metalloproteinase-9 (MMP-9) expression and migration were also increased in response to TNF-alpha in SHR-derived cells. This increase was characterized by the up-regulation of MMP-9, which was transcriptionally regulated at the AP-1 and NF-kappaB sites in the MMP-9 promoter. These results suggest that the hypertensive-associated increase in VSMC proliferative capacity, G1 to S-phase cell-cycle progress and MMP-9 expression may play a role in vascular remodeling in hypertension.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1878-1705
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
837-43
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:19306950-Animals,
pubmed-meshheading:19306950-Aorta,
pubmed-meshheading:19306950-Cell Cycle Proteins,
pubmed-meshheading:19306950-Cell Proliferation,
pubmed-meshheading:19306950-Cell Separation,
pubmed-meshheading:19306950-Cells, Cultured,
pubmed-meshheading:19306950-DNA-Binding Proteins,
pubmed-meshheading:19306950-Flow Cytometry,
pubmed-meshheading:19306950-Gene Expression Regulation,
pubmed-meshheading:19306950-Hypertension,
pubmed-meshheading:19306950-Matrix Metalloproteinase 9,
pubmed-meshheading:19306950-Mutagenesis, Site-Directed,
pubmed-meshheading:19306950-Myocytes, Smooth Muscle,
pubmed-meshheading:19306950-Promoter Regions, Genetic,
pubmed-meshheading:19306950-Protein Binding,
pubmed-meshheading:19306950-Rats,
pubmed-meshheading:19306950-Rats, Inbred WKY,
pubmed-meshheading:19306950-Species Specificity,
pubmed-meshheading:19306950-Tumor Necrosis Factor-alpha,
pubmed-meshheading:19306950-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2009
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pubmed:articleTitle |
TNF-alpha regulates vascular smooth muscle cell responses in genetic hypertension.
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pubmed:affiliation |
Department of Food and Biotechnology, Chungju National University, Chungju, Chungbuk 380-702, South Korea.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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