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rdf:type |
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lifeskim:mentions |
umls-concept:C0007301,
umls-concept:C0021107,
umls-concept:C0033235,
umls-concept:C0040300,
umls-concept:C0041455,
umls-concept:C0043240,
umls-concept:C0086418,
umls-concept:C0225369,
umls-concept:C0374711,
umls-concept:C0439859,
umls-concept:C1441616,
umls-concept:C1705181,
umls-concept:C2603343
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pubmed:issue |
5
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pubmed:dateCreated |
2009-8-31
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pubmed:abstractText |
This study has assessed the relative proportions of type I and II collagens and IIA procollagen in full depth biopsies of repair tissue in a large sample of patients treated with autologous chondrocyte implantation (ACI). Sixty five full depth biopsies were obtained from knees of 58 patients 8-60 months after treatment by ACI alone (n=55) or in combination with mosaicplasty (n=10). In addition articular cartilage was examined from eight individuals (aged 10-50) as controls. Morphology and semi-quantitative immunohistochemistry for collagen types I and II and procollagen IIA in the repair tissue were studied. Repair cartilage thickness was 2.89+/-1.5 mm and there was good basal integration between the repair cartilage, calcified cartilage and subchondral bone. Sixty five percent of the biopsies were predominantly fibrocartilage (mostly type I collagen and IIA procollagen), 15% were hyaline cartilage (mostly type II collagen), 17% were of mixed morphology and 3% were fibrous tissue (mostly type I collagen). Type II collagen and IIA procollagen were usually found in the lower regions near the bone and most type II collagen was present 30-60 months after treatment. The presence of type IIA procollagen in the repair tissue supports our hypothesis that this is indicative of a developing cartilage, with the ratio of type II collagen:procollagen IIA increasing from <2% in the first two years post-treatment to 30% three to five years after treatment. This suggests that cartilage repair tissue produced following ACI treatment, is likely to take some years to mature.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-10085302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-10403272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-10818982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-10845971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-11680679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-11710715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-11879535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-12678357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-12716454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-12804760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-15738679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-15758586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-15855365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-1894696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-2355003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-2918421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-577186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-8078550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-8204907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-9195525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19269183-9358849
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1873-5800
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
398-404
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:19269183-Adult,
pubmed-meshheading:19269183-Biological Markers,
pubmed-meshheading:19269183-Biopsy,
pubmed-meshheading:19269183-Cartilage, Articular,
pubmed-meshheading:19269183-Cell Transplantation,
pubmed-meshheading:19269183-Chondrocytes,
pubmed-meshheading:19269183-Collagen Type I,
pubmed-meshheading:19269183-Collagen Type II,
pubmed-meshheading:19269183-Fibrocartilage,
pubmed-meshheading:19269183-Fluorescent Antibody Technique, Direct,
pubmed-meshheading:19269183-Humans,
pubmed-meshheading:19269183-Immunoenzyme Techniques
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pubmed:year |
2009
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pubmed:articleTitle |
Immunohistochemical study of collagen types I and II and procollagen IIA in human cartilage repair tissue following autologous chondrocyte implantation.
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pubmed:affiliation |
Centre for Spinal Studies, Robert Jones & Agnes Hunt Orthopaedic Hospital, Oswestry, Shropshire, UK. Sally.Roberts@rjah.nhs.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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