Linked Life Data

19269156

Source:http://linkedlifedata.com/resource/pubmed/id/19269156

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-2-3
pubmed:abstractText
The expression and activity of cellular glutathione peroxidase (GPx1) are regulated by selenium (Se). Generally speaking, organic forms of Se have less toxicity and greater bioavailability compared with inorganic forms. In this study, the effects of different forms and concentrations of Se on the regulation of mRNA level and activity of GPx1 in bovine hepatocytes were evaluated, and the optimal doses of different forms of Se that supported the full expression of GPx1 were determined. Primary cultured bovine hepatocyte monolayers derived from neonatal male Holstein calves (aged 1-2 days) were incubated for 24 h with 0 (control), 0.5, 1, 1.5, 2, 3, 4 or 5 micromol/L of Se from dl-selenomethionine (Se-Met), sodium selenite (Na(2)SeO(3)) or Kappa-selenocarrageenan (Se-Car). Compared with controls, a significantly lower level of release of lactic dehydrogenase (LDH) was observed at 0.5-5 micromol/L of Se-Met, 0.5-1 micromol/L of Na(2)SeO(3) and 0.5 micromol/L of Se-Car, but significantly higher LDH release was observed at 2-5 micromol/L of Na(2)SeO(3) and 3-5 micromol/L of Se-Car, and the response occurred in a dose-dependent manner. The intracellular content of reduced glutathione in all hepatocytes treated with Se was significantly lower than that of controls. Significant increases in GPx1 mRNA were obtained in all hepatocytes treated with Se, with maximal effects at 3 micromol/L of Se-Met, 1.5 micromol/L of Na(2)SeO(3) and 2 micromol/L of Se-Car, respectively. Furthermore, 3 micromol/L of Se from Se-Met resulted in peak levels of GPx1 mRNA. After reaching a maximal level, higher Se supplementation led to a reduction of GPx1 mRNA. The activity of GPx1 showed similar patterns but of lower magnitude. We conclude that (a) the regulation of mRNA level and activity of GPx1 in primary cultured bovine hepatocytes by different forms of Se varies and (b) the optimal doses of Se to support the full expression of GPx1 in bovine hepatocytes when supplied as Se-Met, Na(2)SeO(3) and Se-Car are 3, 1.5 and 2 micromol/L, respectively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1873-4847
pubmed:author
pubmed:copyrightInfo
Copyright 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
153-61
pubmed:meshHeading
pubmed-meshheading:19269156-Animals, pubmed-meshheading:19269156-Animals, Newborn, pubmed-meshheading:19269156-Carrageenan, pubmed-meshheading:19269156-Cattle, pubmed-meshheading:19269156-Cell Survival, pubmed-meshheading:19269156-Cells, Cultured, pubmed-meshheading:19269156-Dose-Response Relationship, Drug, pubmed-meshheading:19269156-Glutathione, pubmed-meshheading:19269156-Glutathione Peroxidase, pubmed-meshheading:19269156-Hepatocytes, pubmed-meshheading:19269156-Male, pubmed-meshheading:19269156-Organoselenium Compounds, pubmed-meshheading:19269156-RNA, Messenger, pubmed-meshheading:19269156-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19269156-Selenium, pubmed-meshheading:19269156-Selenomethionine, pubmed-meshheading:19269156-Sodium Selenite, pubmed-meshheading:19269156-Up-Regulation
pubmed:year
2010
pubmed:articleTitle
Regulation of cellular glutathione peroxidase by different forms and concentrations of selenium in primary cultured bovine hepatocytes.
pubmed:affiliation
Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing 210095, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't