Source:http://linkedlifedata.com/resource/pubmed/id/19263498
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2009-4-29
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| pubmed:abstractText |
IL-7 is a cytokine that is required for T-cell development and homeostasis as well as for lymph node organogenesis. Despite the importance of IL-7 in the immune system and its potential therapeutic relevance, questions remain regarding the sites of IL-7 synthesis, specific cell types involved and molecular mechanisms regulating IL-7 expression. To address these issues, we generated two bacterial artificial chromosome (BAC) transgenic mouse lines in which IL-7 regulatory elements drive expression of either Cre recombinase or a human CD25 (hCD25) cell surface reporter molecule. Expression of the IL-7.hCD25 BAC transgene, detected by reactivity with anti-hCD25 antibody, mimicked endogenous IL-7 expression. Fetal and adult tissues from crosses between IL-7.Cre transgenic mice and Rosa26R or R26-EYFP reporters demonstrated X-gal or YFP staining in tissues known to express endogenous IL-7 at some stage during development. These transgenic lines provide novel genetic tools to identify IL-7 producing cells in various tissues and to manipulate gene expression selectively in IL-7 expressing cells.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cre recombinase,
http://linkedlifedata.com/resource/pubmed/chemical/Integrases,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2 Receptor alpha Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-7,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
1526-968X
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
47
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
281-7
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| pubmed:meshHeading |
pubmed-meshheading:19263498-Animals,
pubmed-meshheading:19263498-Animals, Newborn,
pubmed-meshheading:19263498-Chromosomes, Artificial, Bacterial,
pubmed-meshheading:19263498-Female,
pubmed-meshheading:19263498-Flow Cytometry,
pubmed-meshheading:19263498-Gene Expression Profiling,
pubmed-meshheading:19263498-Gene Expression Regulation, Developmental,
pubmed-meshheading:19263498-Humans,
pubmed-meshheading:19263498-Immunohistochemistry,
pubmed-meshheading:19263498-Integrases,
pubmed-meshheading:19263498-Interleukin-2 Receptor alpha Subunit,
pubmed-meshheading:19263498-Interleukin-7,
pubmed-meshheading:19263498-Male,
pubmed-meshheading:19263498-Mice,
pubmed-meshheading:19263498-Mice, Transgenic,
pubmed-meshheading:19263498-Promoter Regions, Genetic,
pubmed-meshheading:19263498-Recombinant Fusion Proteins,
pubmed-meshheading:19263498-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19263498-Thymus Gland
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| pubmed:year |
2009
|
| pubmed:articleTitle |
IL7-hCD25 and IL7-Cre BAC transgenic mouse lines: new tools for analysis of IL-7 expressing cells.
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| pubmed:affiliation |
Department of Carcinogenesis, University of Texas MD Anderson Cancer Center, Science Park Research Division, Smithville, Texas 78957, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|