Linked Life Data

19170032

Source:http://linkedlifedata.com/resource/pubmed/id/19170032

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-1-26
pubmed:abstractText
In clinical settings, chromosome studies are usually performed on solid tissue other than solid tumors for one of two reasons: the tissue biopsy is the only tissue available from the patient, or tissues other than the standard peripheral blood lymphocytes must be examined because of suspected mosaicism. This unit describes methods for culturing tissue samples to be used for preparation of metaphase chromosomes or for biochemical or DNA analysis. Protocols include an efficient enzymatic cell disruption procedure and a mechanical disruption procedure. In addition, a procedure for processing uncultured cells for analysis by fluorescence in situ hybridization (FISH) is provided. Finally, a method is presented that can sometimes be applied to placental tissue involving the use of mitotically active chorionic villus tissue to prepare metaphase chromosome spreads directly, without cell culture. Two support protocols describe methods for collecting tissue samples.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1934-8258
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 8.5
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Preparation and culture of products of conception and other solid tissues for chromosome analysis.
pubmed:affiliation
College of Physicians and Surgeons of Columbia University, New York, USA.
pubmed:publicationType
Journal Article