Linked Life Data

19156359

Source:http://linkedlifedata.com/resource/pubmed/id/19156359

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-5-28
pubmed:abstractText
The tumor microenvironment affects tumor initiation, progression, and metastasis. However, it is still not clear how stromal cells interact with the tumor cells. By using a cytokine array immunoblot assay, we showed that interleukin (IL)-8, IL-6, and RANTES (regulated upon activation normal T-cell expressed and secreted) proteins were up-regulated in GBM8401 glioma cells after coculture with human THP-1-derived macrophages. IL-8 is a chemokine with leukocyte chemotactic, tumorigenic, and proangiogenic properties. To evaluate the correlation of IL-8 expression with tumor-associated macrophages and angiogenesis, 43 glioma specimens were studied. The results showed that the IL-8 mRNA expression and microvessel count in glioma surgical specimens correlated positively with the density of tumor-associated macrophages. We further showed that IL-8 mRNA expression in GBM8401 cells increased dramatically, by 2(8)-2(10)-fold, after being cocultured with macrophages. This increase could also be induced by macrophage-conditioned medium, tumor necrosis factor-alpha, IL-1alpha, and IL-1beta, and could be suppressed by anti-inflammatory agents including pyrrolidine dithiocarbamate, pentoxifylline, or dexamethasone. These findings imply that macrophage infiltration may be the common feature shared by cancer and inflammation, and macrophages could play a role in promoting glioma growth and angiogenesis by inducing IL-8 expression in glioma cells via inflammatory stimuli or the nuclear factor kappa B pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1573-7373
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
289-301
pubmed:meshHeading
pubmed-meshheading:19156359-Brain Neoplasms, pubmed-meshheading:19156359-Chemokine CCL5, pubmed-meshheading:19156359-Coculture Techniques, pubmed-meshheading:19156359-Culture Media, Conditioned, pubmed-meshheading:19156359-Fluorescent Antibody Technique, pubmed-meshheading:19156359-Glioma, pubmed-meshheading:19156359-Humans, pubmed-meshheading:19156359-Immunohistochemistry, pubmed-meshheading:19156359-Interleukin-6, pubmed-meshheading:19156359-Interleukin-8, pubmed-meshheading:19156359-Macrophages, pubmed-meshheading:19156359-Microscopy, Confocal, pubmed-meshheading:19156359-NF-kappa B, pubmed-meshheading:19156359-Neovascularization, Pathologic, pubmed-meshheading:19156359-RNA, Messenger, pubmed-meshheading:19156359-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19156359-Up-Regulation
pubmed:year
2009
pubmed:articleTitle
Induced interleukin-8 expression in gliomas by tumor-associated macrophages.
pubmed:affiliation
NTU Center of Genomic Medicine, National Taiwan University, Taipei, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't