Linked Life Data

19101622

Source:http://linkedlifedata.com/resource/pubmed/id/19101622

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-2-23
pubmed:abstractText
The medicinal herb, Panax notoginseng, has been used for thousands of years in traditional Chinese medicine and possesses anti-fibrosis properties. Epithelial-myofibroblast transition (EMT) plays an important role in renal tubulointerstitial fibrosis. The present study was designed to examine whether ginsenoside Rg1, a major active component isolated from Panax notoginseng, has an ability to block this phenotypic transition in rat renal tubular epithelial cells (NRK-52E) induced by transforming growth factor-beta1 (TGF-beta1). The morphology of tubular epithelial-myofibroblast transition was observed through light microscope and transmission electron microscopy. alpha-SMA and E-cadherin are two markers of tubular epithelial-myofibroblast transition, their protein expressions were assessed by immunohistochemistry and western blot analysis. Gene expression of alpha-SMA as well as the two major extracellular matrix components collagen I and fibronectin was measured by real-time PCR analysis. Enzyme-linked immunosorbent assay was used to quantitatively detect collagen I and fibronectin in the supernatant. Our results revealed that ginsenoside Rg1 obviously blocked morphologic transformation in NRK-52E induced by TGF-beta1. Meanwhile, ginsenoside Rg1 inhibited the expression of alpha-SMA and the loss of E-cadherin, subsequently decreased the levels of collagen I and fibronectin in a dose-dependent manner. In addition, western blot analysis indicated that ginsenoside Rg1 inhibited the expression of P-ERK1/2 in NRK-52E induced by TGF-beta1. These results suggest that ginsenoside Rg1 can restrain the process of EMT maybe via suppressing the expression of P-ERK1/2 in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1872-7573
pubmed:author
pubmed:issnType
Electronic
pubmed:day
25
pubmed:volume
122
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-41
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:19101622-Actins, pubmed-meshheading:19101622-Animals, pubmed-meshheading:19101622-Cadherins, pubmed-meshheading:19101622-Cell Differentiation, pubmed-meshheading:19101622-Cell Line, pubmed-meshheading:19101622-Collagen Type I, pubmed-meshheading:19101622-Epithelial Cells, pubmed-meshheading:19101622-Epithelium, pubmed-meshheading:19101622-Fibroblasts, pubmed-meshheading:19101622-Fibronectins, pubmed-meshheading:19101622-Fibrosis, pubmed-meshheading:19101622-Ginsenosides, pubmed-meshheading:19101622-Kidney Tubules, pubmed-meshheading:19101622-Mitogen-Activated Protein Kinase 3, pubmed-meshheading:19101622-Panax notoginseng, pubmed-meshheading:19101622-Plant Extracts, pubmed-meshheading:19101622-Plant Roots, pubmed-meshheading:19101622-RNA, Messenger, pubmed-meshheading:19101622-Rats, pubmed-meshheading:19101622-Transforming Growth Factor beta1
pubmed:year
2009
pubmed:articleTitle
Ginsenoside Rg1, a major active component isolated from Panax notoginseng, restrains tubular epithelial to myofibroblast transition in vitro.
pubmed:affiliation
Department of Nephrology, West China Hospital of Sichuan University, No. 37, Guoxuexiang, Wuhou, Chengdu 610041, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't