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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2009-1-30
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pubmed:abstractText |
Currently the confocal scanning microscopy of fluorescently tagged molecules is extensively employed to acquire quantitative data on gene expression at cellular resolution. Following this approach, we generated a large dataset on the expression of segmentation genes in the Drosophila blastoderm, that is widely used in systems biology studies. As data accuracy is of critical importance for the success of studies in this field, we took a shot to evaluate possible errors introduced in the data by acquisition and processing methods. This article deals with errors introduced by confocal microscope.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19052059-10556096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19052059-11500845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19052059-11500846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19052059-12801865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19052059-15059816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19052059-15059825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19052059-15834586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19052059-16586486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19052059-18238264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19052059-2901040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19052059-6411748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19052059-9683745
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1367-4811
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
346-52
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pubmed:dateRevised |
2010-9-23
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pubmed:meshHeading |
pubmed-meshheading:19052059-Animals,
pubmed-meshheading:19052059-Blastoderm,
pubmed-meshheading:19052059-Drosophila,
pubmed-meshheading:19052059-Gene Expression,
pubmed-meshheading:19052059-Gene Expression Profiling,
pubmed-meshheading:19052059-Genes, Insect,
pubmed-meshheading:19052059-Image Processing, Computer-Assisted,
pubmed-meshheading:19052059-Microscopy, Confocal,
pubmed-meshheading:19052059-Regression Analysis
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pubmed:year |
2009
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pubmed:articleTitle |
Estimation of errors introduced by confocal imaging into the data on segmentation gene expression in Drosophila.
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pubmed:affiliation |
St Petersburg State Polytechnic University, St Petersburg, 195251, Russia. myasnikova@spbcas.ru
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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