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rdf:type |
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lifeskim:mentions |
umls-concept:C0001721,
umls-concept:C0017262,
umls-concept:C0086418,
umls-concept:C0225369,
umls-concept:C0225371,
umls-concept:C0521339,
umls-concept:C0871261,
umls-concept:C1456820,
umls-concept:C1512840,
umls-concept:C1533691,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
3
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pubmed:dateCreated |
2008-12-19
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pubmed:abstractText |
Cartilage-specific extracellular matrix synthesis is the prerequisite for chondrocyte survival and cartilage function, but is affected by the pro-inflammatory cytokine TNF-alpha in arthritis. The aim of the present study was to characterize whether the immunoregulatory cytokine IL-10 might modulate cartilage matrix and cytokine expression in response to TNF-alpha. Primary human articular chondrocytes were treated with either recombinant IL-10, TNF-alpha or a combination of both (at 10ng/mL each) or transduced with an adenoviral vector overexpressing human IL-10 and subsequently stimulated with 10ng/ml TNF-alpha for 6 or 24h. The effects of IL-10 on the cartilage-specific matrix proteins collagen type II, aggrecan, matrix-metalloproteinases (MMP)-3, -13 and pro-inflammatory cytokines were evaluated by real-time RT-PCR and immunohistochemistry. Transduced chondrocytes overexpressed high levels of IL-10 which significantly up-regulated collagen type II expression. TNF-alpha suppressed collagen type II and aggrecan, but increased MMP and cytokine expression in chondrocytes compared to the non-stimulated controls. The TNF-alpha mediated down-regulation of aggrecan expression was significantly antagonized by IL-10 overexpression, whereas the suppression of collagen type II was barely affected. The MMP-13 and IL-1beta expression by TNF-alpha was slightly reduced by IL-10. These results suggest that IL-10 overexpression modulates some catabolic features of TNF-alpha in chondrocytes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1096-0023
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pubmed:author |
pubmed-author:ErtelWW,
pubmed-author:GuteDD,
pubmed-author:HellmuthMM,
pubmed-author:HostmannAA,
pubmed-author:HutchinsBB,
pubmed-author:JohnTT,
pubmed-author:KohlBB,
pubmed-author:LafaceDD,
pubmed-author:LaubeGG,
pubmed-author:MüllerR DRD,
pubmed-author:OberholzerAA,
pubmed-author:Schulze-TanzilGG,
pubmed-author:TschoekeS KSK,
pubmed-author:VehR WRW
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pubmed:issnType |
Electronic
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
377-85
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pubmed:meshHeading |
pubmed-meshheading:19026560-Adenoviridae,
pubmed-meshheading:19026560-Aged,
pubmed-meshheading:19026560-Aged, 80 and over,
pubmed-meshheading:19026560-Arteries,
pubmed-meshheading:19026560-Cells, Cultured,
pubmed-meshheading:19026560-Chondrocytes,
pubmed-meshheading:19026560-Collagen Type II,
pubmed-meshheading:19026560-Extracellular Matrix Proteins,
pubmed-meshheading:19026560-Gene Expression Regulation,
pubmed-meshheading:19026560-Genetic Vectors,
pubmed-meshheading:19026560-Glycoproteins,
pubmed-meshheading:19026560-Humans,
pubmed-meshheading:19026560-Interleukin-10,
pubmed-meshheading:19026560-Matrix Metalloproteinase 13,
pubmed-meshheading:19026560-Matrix Metalloproteinase 3,
pubmed-meshheading:19026560-Microscopy, Electron, Transmission,
pubmed-meshheading:19026560-Middle Aged,
pubmed-meshheading:19026560-RNA, Messenger,
pubmed-meshheading:19026560-Tumor Necrosis Factor-alpha
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pubmed:year |
2008
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pubmed:articleTitle |
IL-10 overexpression differentially affects cartilage matrix gene expression in response to TNF-alpha in human articular chondrocytes in vitro.
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pubmed:affiliation |
Department of Trauma and Reconstructive Surgery, Charité-University of Medicine, Campus Benjamin Franklin, FEM, Krahmerstr. 6-10, 12207 Berlin, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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