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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
47
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pubmed:dateCreated |
2008-11-26
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pubmed:abstractText |
Combinatorial nuclear transformation is a novel method for the rapid production of multiplex-transgenic plants, which we have used to dissect and modify a complex metabolic pathway. To demonstrate the principle, we transferred 5 carotenogenic genes controlled by different endosperm-specific promoters into a white maize variety deficient for endosperm carotenoid synthesis. We recovered a diverse population of transgenic plants expressing different enzyme combinations and showing distinct metabolic phenotypes that allowed us to identify and complement rate-limiting steps in the pathway and to demonstrate competition between beta-carotene hydroxylase and bacterial beta-carotene ketolase for substrates in 4 sequential steps of the extended pathway. Importantly, this process allowed us to generate plants with extraordinary levels of beta-carotene and other carotenoids, including complex mixtures of hydroxycarotenoids and ketocarotenoids. Combinatorial transformation is a versatile approach that could be used to modify any metabolic pathway and pathways controlling other biochemical, physiological, or developmental processes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-10634784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-11131010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-11361022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-11805345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-12437084,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-12897253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-14704330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-15003396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-15081054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-15247400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-15272869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-15533882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-15659105,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-16007373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-16307363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-16565214,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-17265153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-17406674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-17608668,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-8275101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-8722797,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19011084-9523693
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1091-6490
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
25
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pubmed:volume |
105
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
18232-7
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pubmed:dateRevised |
2010-9-23
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pubmed:meshHeading |
pubmed-meshheading:19011084-Carotenoids,
pubmed-meshheading:19011084-Genes, Plant,
pubmed-meshheading:19011084-Mixed Function Oxygenases,
pubmed-meshheading:19011084-Mutation,
pubmed-meshheading:19011084-Oxygenases,
pubmed-meshheading:19011084-Plants, Genetically Modified,
pubmed-meshheading:19011084-Substrate Specificity,
pubmed-meshheading:19011084-Transformation, Genetic,
pubmed-meshheading:19011084-Zea mays
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pubmed:year |
2008
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pubmed:articleTitle |
Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize.
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pubmed:affiliation |
Departament de Producció Vegetal i Ciència Forestal, Universitat de Lleida, Avenue Alcalde Rovira Roure, 191, 25198 Lleida, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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