Source:http://linkedlifedata.com/resource/pubmed/id/18854944
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0004927,
umls-concept:C0005821,
umls-concept:C0022022,
umls-concept:C0024264,
umls-concept:C0109317,
umls-concept:C0596235,
umls-concept:C0596901,
umls-concept:C0752312,
umls-concept:C1150579,
umls-concept:C1333340,
umls-concept:C1366882,
umls-concept:C1370600,
umls-concept:C1550548,
umls-concept:C1555714,
umls-concept:C1705654,
umls-concept:C1705767,
umls-concept:C1705791,
umls-concept:C1879547
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| pubmed:issue |
23
|
| pubmed:dateCreated |
2008-12-4
|
| pubmed:abstractText |
Rapid Ca2+-dependent phospholipid (PL) reorganization (scrambling) at the plasma membrane is a mechanism common to hematopoietic cells exposing procoagulant phosphatidylserine (PS). The aim of this research was to determine whether activation of the extracellular signal-regulated kinase (ERK) pathway was required for PL scrambling, based on a single report analyzing both responses induced by Ca2+ ionophores in megakaryoblastic HEL cells. Ca2+ ionophore-stimulated ERK phosphorylation was induced in platelets without external Ca2+, whereas exogenous Ca2+ entry was crucial for ERK activation in Jurkat T cells. In both cells, membrane scrambling only occurred following Ca2+ entry and was not blocked by inhibiting ERK phosphorylation. Furthermore, ERK proteins are strongly phosphorylated in transformed B lymphoblastic cell lines, which do not expose PS in their resting state. Overall, the data demonstrated that ERK activation and membrane scrambling are independent mechanisms.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
1420-9071
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| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:volume |
65
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3861-71
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:18854944-Blood Platelets,
pubmed-meshheading:18854944-Blotting, Western,
pubmed-meshheading:18854944-Calcium,
pubmed-meshheading:18854944-Cell Membrane,
pubmed-meshheading:18854944-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:18854944-Flow Cytometry,
pubmed-meshheading:18854944-Humans,
pubmed-meshheading:18854944-Ionophores,
pubmed-meshheading:18854944-Jurkat Cells,
pubmed-meshheading:18854944-Lymphocytes,
pubmed-meshheading:18854944-Microscopy, Fluorescence,
pubmed-meshheading:18854944-Phospholipids,
pubmed-meshheading:18854944-Phosphorylation,
pubmed-meshheading:18854944-Signal Transduction
|
| pubmed:year |
2008
|
| pubmed:articleTitle |
ERK activation by Ca2+ ionophores depends on Ca2+ entry in lymphocytes but not in platelets, and does not conduct membrane scrambling.
|
| pubmed:affiliation |
INSERM U770 and Université Paris-Sud, 80 rue du Général Leclerc, 94276 Le Kremlin-Bicêtre cedex, France.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|