Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
2008-10-17
pubmed:abstractText
Normal and modified urinary nucleosides represent potential biomarkers for cancer diagnosis. To selectively extract modified nucleosides, we developed a molecularly imprinted polymer (MIP) of 5-methyluridine as selective material for molecularly imprinted solid-phase extraction (MISPE). The MIPs were obtained from vinyl-phenylboronate ester derivative of the template, acrylamide and pentaerythritol triacrylate co-polymer, and were tested in batch and cartridge experiments with aqueous samples. Our results indicated that the imprinted polymer was selective for pyrimidine nucleosides with a K(d) and a B(max) of 46 microM and 18 micromol/g, respectively. Finally, a MISPE of the most common pyrimidine nucleoside cancer markers in urine sample was realized.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1464-3391
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8932-9
pubmed:meshHeading
pubmed-meshheading:18789867-Acrylamide, pubmed-meshheading:18789867-Acrylates, pubmed-meshheading:18789867-Animals, pubmed-meshheading:18789867-Biological Markers, pubmed-meshheading:18789867-Chromatography, High Pressure Liquid, pubmed-meshheading:18789867-Dogs, pubmed-meshheading:18789867-Mass Spectrometry, pubmed-meshheading:18789867-Microscopy, Electron, Scanning, pubmed-meshheading:18789867-Molecular Imprinting, pubmed-meshheading:18789867-Neoplasms, pubmed-meshheading:18789867-Polymers, pubmed-meshheading:18789867-Propylene Glycols, pubmed-meshheading:18789867-Pyrimidine Nucleosides, pubmed-meshheading:18789867-Reproducibility of Results, pubmed-meshheading:18789867-Sensitivity and Specificity, pubmed-meshheading:18789867-Solid Phase Extraction, pubmed-meshheading:18789867-Uridine, pubmed-meshheading:18789867-Vinyl Compounds
pubmed:year
2008
pubmed:articleTitle
Molecularly imprinted polymer of 5-methyluridine for solid-phase extraction of pyrimidine nucleoside cancer markers in urine.
pubmed:affiliation
Institut de Chimie Organique et Analytique (ICOA), CNRS UMR 6005, Université d'Orléans, BP 6759, Orléans 45067 Cedex 2, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't