rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
19
|
pubmed:dateCreated |
2008-10-17
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pubmed:abstractText |
Normal and modified urinary nucleosides represent potential biomarkers for cancer diagnosis. To selectively extract modified nucleosides, we developed a molecularly imprinted polymer (MIP) of 5-methyluridine as selective material for molecularly imprinted solid-phase extraction (MISPE). The MIPs were obtained from vinyl-phenylboronate ester derivative of the template, acrylamide and pentaerythritol triacrylate co-polymer, and were tested in batch and cartridge experiments with aqueous samples. Our results indicated that the imprinted polymer was selective for pyrimidine nucleosides with a K(d) and a B(max) of 46 microM and 18 micromol/g, respectively. Finally, a MISPE of the most common pyrimidine nucleoside cancer markers in urine sample was realized.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Oct
|
pubmed:issn |
1464-3391
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pubmed:author |
|
pubmed:issnType |
Electronic
|
pubmed:day |
1
|
pubmed:volume |
16
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
8932-9
|
pubmed:meshHeading |
pubmed-meshheading:18789867-Acrylamide,
pubmed-meshheading:18789867-Acrylates,
pubmed-meshheading:18789867-Animals,
pubmed-meshheading:18789867-Biological Markers,
pubmed-meshheading:18789867-Chromatography, High Pressure Liquid,
pubmed-meshheading:18789867-Dogs,
pubmed-meshheading:18789867-Mass Spectrometry,
pubmed-meshheading:18789867-Microscopy, Electron, Scanning,
pubmed-meshheading:18789867-Molecular Imprinting,
pubmed-meshheading:18789867-Neoplasms,
pubmed-meshheading:18789867-Polymers,
pubmed-meshheading:18789867-Propylene Glycols,
pubmed-meshheading:18789867-Pyrimidine Nucleosides,
pubmed-meshheading:18789867-Reproducibility of Results,
pubmed-meshheading:18789867-Sensitivity and Specificity,
pubmed-meshheading:18789867-Solid Phase Extraction,
pubmed-meshheading:18789867-Uridine,
pubmed-meshheading:18789867-Vinyl Compounds
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pubmed:year |
2008
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pubmed:articleTitle |
Molecularly imprinted polymer of 5-methyluridine for solid-phase extraction of pyrimidine nucleoside cancer markers in urine.
|
pubmed:affiliation |
Institut de Chimie Organique et Analytique (ICOA), CNRS UMR 6005, Université d'Orléans, BP 6759, Orléans 45067 Cedex 2, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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