Linked Life Data

1868983

Source:http://linkedlifedata.com/resource/pubmed/id/1868983

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pubmed-article:1868983pubmed:abstractTextHistamine stimulation of swine arterial smooth muscle is associated with a high [Ca2+]i sensitivity for increases in myosin light-chain phosphorylation. In contrast, KCl depolarization produces a relatively lower [Ca2+]i sensitivity (i.e., similar increases in [Ca2+]i induce less myosin phosphorylation). We evaluated whether 1) artifacts in the methodology for measuring [Ca2+]i or 2) true alterations in the [Ca2+]i sensitivity of myosin light-chain kinase were responsible for these apparent changes in the [Ca2+]i sensitivity of phosphorylation. The [Ca2+]i sensitivity of phosphorylation was higher with histamine stimulation regardless of whether the [Ca2+]i indicator was aequorin (which was loaded intracellularly by reversible hyperpermeabilization) or Fura 2 (which was loaded intracellularly by incubation of the tissues in Fura 2 AM). Aequorin and Fura 2 appeared to detect qualitatively similar stimulus-induced changes in [Ca2+]i with the exception that the initial response to histamine stimulation was different (histamine initially induced a large aequorin light transient and a relatively smaller increase in Fura 2 fluorescence). The [Ca2+]i sensitivity of myosin light-chain kinase extracted from KCl depolarized tissues was lower than the [Ca2+]i sensitivity of myosin light-chain kinase extracted from unstimulated or histamine stimulated tissues. These results suggest that depolarization specifically modifies myosin light-chain kinase to decrease its [Ca2+]i sensitivity. Changes in the [Ca2+]i sensitivity of myosin light-chain phosphorylation are not an artifact of the [Ca2+]i measurement technique.lld:pubmed
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pubmed-article:1868983pubmed:authorpubmed-author:WeaverB ABAlld:pubmed
pubmed-article:1868983pubmed:authorpubmed-author:RemboldC MCMlld:pubmed
pubmed-article:1868983pubmed:authorpubmed-author:GilbertE KEKlld:pubmed
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pubmed-article:1868983pubmed:pagination2593-9lld:pubmed
pubmed-article:1868983pubmed:dateRevised2009-11-19lld:pubmed
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pubmed-article:1868983pubmed:year1991lld:pubmed
pubmed-article:1868983pubmed:articleTitleDepolarization decreases the [Ca2+]i sensitivity of myosin light-chain kinase in arterial smooth muscle: comparison of aequorin and fura 2 [Ca2+]i estimates.lld:pubmed
pubmed-article:1868983pubmed:affiliationDepartment of Internal Medicine, University of Virginia Health Science Center, Charlottesville 22908.lld:pubmed
pubmed-article:1868983pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1868983pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:1868983pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:1868983pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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