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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1991-9-17
|
| pubmed:abstractText |
This report describes a rapid, reproducible in vitro bioassay to quantitate the cytotoxic activity of human tumor necrosis factor-alpha using a human rather than murine cell line in the absence of metabolic inhibitors. The target cells are BT-20 (breast carcinoma) cultured at 39 degrees C in the presence of recombinant human tumor necrosis factor-alpha (rHuTNF-alpha) in 96-well microtiter plates for 2 days. Cytotoxicity is measured by the crystal violet dye uptake of the remaining viable cells. This bioassay is sensitive to 1.5 ng/ml of rHuTNF-alpha, with an assay range to 130 ng/ml. Samples spiked into human plasma are measurable from 0.5 to 150 ng/ml. The specificity of this cytotoxic effect on the BT-20 cell line was demonstrated using rHuTNF-alpha neutralizing antibodies. A panel of cytokines including interferons, interleukins, and tumor necrosis factors was also analyzed using this assay system. Of the cytokines assayed, only recombinant murine tumor necrosis factor-alpha and recombinant human tumor necrosis factor-beta demonstrated measurable cytotoxic activity when assayed independently, while recombinant human interferon-gamma was the only cytokine to demonstrate greater than additive activity in combination with rHuTNF-alpha. The simplicity and reproducibility of this assay on a human cell line makes it useful for the routine determination of the biological activity of human tumor necrosis factor-alpha.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
1053-8550
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
10
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
174-81
|
| pubmed:dateRevised |
2008-3-18
|
| pubmed:meshHeading |
pubmed-meshheading:1868041-Antigen-Antibody Reactions,
pubmed-meshheading:1868041-Biological Assay,
pubmed-meshheading:1868041-Cell Division,
pubmed-meshheading:1868041-Drug Screening Assays, Antitumor,
pubmed-meshheading:1868041-Humans,
pubmed-meshheading:1868041-Reproducibility of Results,
pubmed-meshheading:1868041-Time Factors,
pubmed-meshheading:1868041-Tumor Cells, Cultured,
pubmed-meshheading:1868041-Tumor Necrosis Factor-alpha
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
A rapid in vitro cytotoxicity assay for the detection of tumor necrosis factor on human BT-20 cells.
|
| pubmed:affiliation |
Medicinal and Analytical Chemistry, Genentech, Inc., South San Francisco, CA 94080.
|
| pubmed:publicationType |
Journal Article
|