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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
41
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pubmed:dateCreated |
2008-10-6
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pubmed:abstractText |
The receptor tyrosine kinase Ror2 has recently been shown to act as an alternative receptor or coreceptor for Wnt5a and to mediate Wnt5a-induced migration of cultured cells. However, little is known about the molecular mechanism underlying this migratory process. Here we show by wound-healing assays that Ror2 plays critical roles in Wnt5a-induced cell migration by regulating formation of lamellipodia and reorientation of microtubule-organizing center (MTOC). Wnt5a stimulation induces activation of the c-Jun N-terminal kinase JNK at the wound edge in a Ror2-dependent manner, and inhibiting JNK activity abrogates Wnt5a-induced lamellipodia formation and MTOC reorientation. Additionally, the association of Ror2 with the actin-binding protein filamin A is required for Wnt5a-induced JNK activation and polarized cell migration. We further show that Wnt5a-induced JNK activation and MTOC reorientation can be suppressed by inhibiting PKCzeta. Taken together, our findings indicate that Wnt5a/Ror2 activates JNK, through a process involving filamin A and PKCzeta, to regulate polarized cell migration.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Contractile Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Tyrosine Kinase-like...,
http://linkedlifedata.com/resource/pubmed/chemical/Ror2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt5a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/filamins,
http://linkedlifedata.com/resource/pubmed/chemical/protein kinase C zeta
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
283
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
27973-81
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:18667433-Actins,
pubmed-meshheading:18667433-Animals,
pubmed-meshheading:18667433-Cell Movement,
pubmed-meshheading:18667433-Cell Polarity,
pubmed-meshheading:18667433-Contractile Proteins,
pubmed-meshheading:18667433-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:18667433-Mice,
pubmed-meshheading:18667433-Microfilament Proteins,
pubmed-meshheading:18667433-Microtubule-Organizing Center,
pubmed-meshheading:18667433-NIH 3T3 Cells,
pubmed-meshheading:18667433-Protein Kinase C,
pubmed-meshheading:18667433-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:18667433-Receptor Tyrosine Kinase-like Orphan Receptors,
pubmed-meshheading:18667433-Wnt Proteins,
pubmed-meshheading:18667433-Wound Healing
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pubmed:year |
2008
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pubmed:articleTitle |
Receptor tyrosine kinase Ror2 mediates Wnt5a-induced polarized cell migration by activating c-Jun N-terminal kinase via actin-binding protein filamin A.
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pubmed:affiliation |
Department of Physiology and Cell Biology, Graduate School of Medicine, Kobe University, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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