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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2008-9-15
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pubmed:abstractText |
Gene expression analysis on laser capture microdissected samples can be hampered because of the small sample size. The interference of PCR inhibitors increases with smaller sample size. Real-time reverse transcription PCR (RT-PCR) is usually performed directly after the reverse transcription step, enabling PCR inhibitors to remain in the complementary DNA (cDNA). A protocol was optimized for real-time RT-PCR with SYBR Green I. The introduction of an additional cDNA purification step after reverse transcription removed PCR inhibitors, making the reaction more efficient.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1096-0309
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
382
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
72-4
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
Elimination of amplification artifacts in real-time reverse transcription PCR using laser capture microdissected samples.
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pubmed:affiliation |
Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. ward.despiegelaere@ugent.be
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pubmed:publicationType |
Journal Article
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