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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2008-8-25
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pubmed:abstractText |
Surface modifications of poly(lactide-co-glycolide) microparticles with different polycationic electrolytes have mainly been studied for conjugation to antigens and/or adjuvants. However, the in vivo immunological effects of using surface charged particles have not been address yet. In this study, microparticles were coated or not with protamine, a cationic and arginine-rich electrolyte that confers microparticles with a positively surface charge. We then evaluated the potential of protamine-coatings to assist the induction of immune responses in mice. Interestingly, enhanced antibodies and T-cell responses were observed in mice treated with the coated particles. In vitro studies suggested that the improved immunological performance was mediated by an increased uptake. Indeed, protamine-coated particles that carried a plasmid were even internalised into non-phagocytic cells and to cause their transfection. These results open the way for further research into a novel technology that combines the use protamine for facilitated cell penetration of that and biodegradable microparticles for prolonged antigen or drug release.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Carriers,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lactic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Polyamines,
http://linkedlifedata.com/resource/pubmed/chemical/Polyglycolic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Protamines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/polycations,
http://linkedlifedata.com/resource/pubmed/chemical/polylactic acid-polyglycolic acid...
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1873-4995
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
10
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pubmed:volume |
130
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
161-7
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pubmed:meshHeading |
pubmed-meshheading:18588928-Animals,
pubmed-meshheading:18588928-Antibody Formation,
pubmed-meshheading:18588928-Antigens,
pubmed-meshheading:18588928-B-Lymphocytes,
pubmed-meshheading:18588928-Cell Line,
pubmed-meshheading:18588928-DNA,
pubmed-meshheading:18588928-Dendritic Cells,
pubmed-meshheading:18588928-Drug Carriers,
pubmed-meshheading:18588928-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:18588928-Female,
pubmed-meshheading:18588928-Flow Cytometry,
pubmed-meshheading:18588928-Green Fluorescent Proteins,
pubmed-meshheading:18588928-Humans,
pubmed-meshheading:18588928-Lactic Acid,
pubmed-meshheading:18588928-Mice,
pubmed-meshheading:18588928-Mice, Inbred BALB C,
pubmed-meshheading:18588928-Mice, Inbred C57BL,
pubmed-meshheading:18588928-Mice, Inbred CBA,
pubmed-meshheading:18588928-Mice, Transgenic,
pubmed-meshheading:18588928-Particle Size,
pubmed-meshheading:18588928-Phagocytosis,
pubmed-meshheading:18588928-Polyamines,
pubmed-meshheading:18588928-Polyglycolic Acid,
pubmed-meshheading:18588928-Protamines,
pubmed-meshheading:18588928-Receptors, Antigen, T-Cell,
pubmed-meshheading:18588928-Surface Properties,
pubmed-meshheading:18588928-T-Lymphocytes,
pubmed-meshheading:18588928-Transfection
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pubmed:year |
2008
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pubmed:articleTitle |
Surface coating of PLGA microparticles with protamine enhances their immunological performance through facilitated phagocytosis.
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pubmed:affiliation |
Unit for Experimental Immunotherapy, Department of Dermatology, University Hospital Zurich, Gloriastrasse 31, 8091 Zurich, Switzerland.
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pubmed:publicationType |
Journal Article
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