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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
2008-6-30
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pubmed:abstractText |
In recent years stable isotopic labeling has become a standard approach for quantitative proteomic analyses. Among the many available isotopic labeling strategies, metabolic labeling is attractive for the excellent internal control it provides. However, analysis of data from metabolic labeling experiments can be complicated because the spacing between labeled and unlabeled forms of each peptide depends on its sequence, and is thus variable from analyte to analyte. As a result, one generally needs to know the sequence of a peptide to identify its matching isotopic distributions in an automated fashion. In some experimental situations it would be necessary or desirable to match pairs of labeled and unlabeled peaks from peptides of unknown sequence. This article addresses this largely overlooked problem in the analysis of quantitative mass spectrometry data by presenting an algorithm that not only identifies isotopic distributions within a mass spectrum, but also annotates matches between natural abundance light isotopic distributions and their metabolically labeled counterparts. This algorithm is designed in two stages: first we annotate the isotopic peaks using a modified version of the IDM algorithm described last year; then we use a probabilistic classifier that is supplemented by dynamic programming to find the metabolically labeled matched isotopic pairs. Such a method is needed for high-throughput quantitative proteomic metabolomic experiments measured via mass spectrometry.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-10424174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-10504701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-10612281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-10757168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-11581660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-11840562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-12356725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-12570156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-12590263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-12748939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-12832456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-12832459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-12837591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-12837596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-12858183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-15385600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-15595724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-15789030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-15822913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-15822937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-15849272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-15924362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-16053308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-16458531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-16614208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-17293592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-17443642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-17586823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-17646314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18586733-17708672
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1367-4811
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
i339-47
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
Matching isotopic distributions from metabolically labeled samples.
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pubmed:affiliation |
Department of Computer Sciences, University of Wisconsin, Madison, WI, USA. mcilwain@cs.wisc.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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