Source:http://linkedlifedata.com/resource/pubmed/id/18577714
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2008-8-19
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| pubmed:abstractText |
Monocytes of bone marrow (BM) origin are circulating precursors that replenish dendritic cells and macrophage populations in peripheral tissues during homeostasis. The eye provides a unique range of varying tissue microenvironments in which to compare the different turnover rates of monocyte-derived cells. This was investigated in the present study using radiation chimeras, whereby BM from Cx3cr1(+/gfp) mice was used to rescue myeloablated wild-type (WT) BALB/c mice (conventional chimeras). The use of Cx3cr1(+/gfp) mice as BM donors allowed the clear visualization of newly recruited monocyte-derived cells. Following BM reconstitution, mice were killed at 2, 4, 6, and 8 weeks, and wholemount ocular tissues were processed for immunohistochemistry and confocal microscopy. "Reverse" chimeras (WT into Cx3cr1(+/gfp)) were also created to act as a further method of cross-referencing cell turnover rates. In conventional chimeras, Cx3cr1(+/gfp) cells began repopulating the uveal tract (iris, ciliary body, choroid) 2 weeks post-transplantation with close to complete replenishment by 8 weeks. By contrast, the earliest recruitment of Cx3cr1(+/gfp) cells into the host retina occurred at 4 weeks. In reverse chimeras, a steady accumulation of host Cx3cr1(+/gfp) macrophages in the subretinal space of Cx3cr1(+/gfp) adult mice suggests that these cells arise from long-term resident microglia and not newly recruited WT donor cells. In summary, chimeric mouse models, in which lineage-specific cells carry a fluorescent reporter, have been used in the present study to visualize the turnover of monocyte-derived cells in different tissue compartments of the eye. These data provide valuable insights into differential monocyte turnover rates within a single complex organ.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cx3cr1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Chemokine,
http://linkedlifedata.com/resource/pubmed/chemical/enhanced green fluorescent protein
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0741-5400
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
84
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
721-9
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| pubmed:meshHeading |
pubmed-meshheading:18577714-Animals,
pubmed-meshheading:18577714-Bone Marrow,
pubmed-meshheading:18577714-Cell Lineage,
pubmed-meshheading:18577714-Ciliary Body,
pubmed-meshheading:18577714-Dendritic Cells,
pubmed-meshheading:18577714-Eye,
pubmed-meshheading:18577714-Female,
pubmed-meshheading:18577714-Green Fluorescent Proteins,
pubmed-meshheading:18577714-Iris,
pubmed-meshheading:18577714-Macrophages,
pubmed-meshheading:18577714-Mice,
pubmed-meshheading:18577714-Mice, Inbred BALB C,
pubmed-meshheading:18577714-Mice, Transgenic,
pubmed-meshheading:18577714-Microglia,
pubmed-meshheading:18577714-Monocytes,
pubmed-meshheading:18577714-Radiation Chimera,
pubmed-meshheading:18577714-Receptors, Chemokine,
pubmed-meshheading:18577714-Retina,
pubmed-meshheading:18577714-Uvea
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| pubmed:year |
2008
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| pubmed:articleTitle |
Differential turnover rates of monocyte-derived cells in varied ocular tissue microenvironments.
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| pubmed:affiliation |
School of Anatomy and Human Biology, The University of Western Australia, Perth, Western Australia.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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