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rdf:type |
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lifeskim:mentions |
umls-concept:C0001884,
umls-concept:C0014264,
umls-concept:C0017262,
umls-concept:C0024432,
umls-concept:C0026809,
umls-concept:C0036974,
umls-concept:C0054434,
umls-concept:C0079904,
umls-concept:C0109317,
umls-concept:C0185117,
umls-concept:C0387583,
umls-concept:C0752312,
umls-concept:C1150579,
umls-concept:C1333340,
umls-concept:C1366876,
umls-concept:C1366882,
umls-concept:C1370600,
umls-concept:C1533698,
umls-concept:C1704259,
umls-concept:C1705767,
umls-concept:C1705791,
umls-concept:C1705987,
umls-concept:C1709843,
umls-concept:C1833235,
umls-concept:C2911684
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pubmed:issue |
11
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pubmed:dateCreated |
2008-8-5
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pubmed:abstractText |
Caffeic acid phenethyl ester has been shown to have anti-inflammatory and anti-cancer effects. We examined the effects of caffeic acid phenethyl ester on lipopolysaccharide-induced production of nitric oxide and prostaglandin E2, and expression of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 macrophages. We also investigated the effects of caffeic acid phenethyl ester on lipopolysaccharide-induced septic shock in mice. Our results indicate that caffeic acid phenethyl ester inhibits lipopolysaccharide-induced nitric oxide and prostaglandin E2 production in a concentration-dependent manner and inhibits inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 cells, without significant cytotoxicity. To further examine the mechanism responsible for the inhibition of inducible nitric oxide synthase and cyclooxygenase-2 expression by caffeic acid phenethyl ester, we examined the effect of caffeic acid phenethyl ester on lipopolysaccharide-induced nuclear factor-kappaB activation and the phosphorylation of mitogen-activated protein kinases. Caffeic acid phenethyl ester treatment significantly reduced nuclear factor-kappaB translocation and DNA-binding in lipopolysaccharide-stimulated RAW 264.7 cells. This effect was mediated through the inhibition of the degradation of inhibitor kappaB and by inhibition of both p38 mitogen-activated protein kinase and extracellular signal-regulated kinase phosphorylation, at least in part by inhibiting the generation of reactive oxygen species. Furthermore, caffeic acid phenethyl ester rescued C57BL/6 mice from lethal lipopolysaccharide-induced septic shock, while decreasing serum levels of tumor necrosis factor-alpha and interleukin-1beta. Collectively, these results suggest that caffeic acid phenethyl ester suppresses the induction of cytokines by lipopolysaccharide, as well as inducible nitric oxide synthase and cyclooxygenase-2 expression, by blocking nuclear factor-kappaB and p38/ERK activation. These findings provide mechanistic insights into the anti-inflammatory and chemopreventive actions of caffeic acid phenethyl ester in macrophages.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caffeic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1beta,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylethyl Alcohol,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/caffeic acid phenethyl ester,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
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pubmed:status |
MEDLINE
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pubmed:issn |
1357-2725
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pubmed:author |
pubmed-author:ChoiIl-WhanIW,
pubmed-author:ChoiInhakI,
pubmed-author:ChoiYung HyunYH,
pubmed-author:JungWon-KyoWK,
pubmed-author:KimMoon-MooMM,
pubmed-author:LeeChang-MinCM,
pubmed-author:LeeDa-YoungDY,
pubmed-author:LeeSoo-WoongSW,
pubmed-author:ParkSae-GwangSG,
pubmed-author:ParkYeong-MinYM,
pubmed-author:SeoSu-KilSK,
pubmed-author:YeaSung SuSS
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pubmed:issnType |
Print
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pubmed:volume |
40
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2572-82
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:18571461-Animals,
pubmed-meshheading:18571461-Caffeic Acids,
pubmed-meshheading:18571461-Cell Line,
pubmed-meshheading:18571461-Cell Survival,
pubmed-meshheading:18571461-Cyclooxygenase 2,
pubmed-meshheading:18571461-Dinoprostone,
pubmed-meshheading:18571461-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:18571461-Interleukin-1beta,
pubmed-meshheading:18571461-Lipopolysaccharides,
pubmed-meshheading:18571461-Macrophages,
pubmed-meshheading:18571461-Mice,
pubmed-meshheading:18571461-Molecular Structure,
pubmed-meshheading:18571461-NF-kappa B,
pubmed-meshheading:18571461-Nitric Oxide,
pubmed-meshheading:18571461-Nitric Oxide Synthase Type II,
pubmed-meshheading:18571461-Phenylethyl Alcohol,
pubmed-meshheading:18571461-Shock, Septic,
pubmed-meshheading:18571461-Signal Transduction,
pubmed-meshheading:18571461-Tumor Necrosis Factor-alpha,
pubmed-meshheading:18571461-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2008
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pubmed:articleTitle |
Caffeic acid phenethyl ester protects mice from lethal endotoxin shock and inhibits lipopolysaccharide-induced cyclooxygenase-2 and inducible nitric oxide synthase expression in RAW 264.7 macrophages via the p38/ERK and NF-kappaB pathways.
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pubmed:affiliation |
Department of Marine Life Science, Chosun University, Gwangju 501-759, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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