Source:http://linkedlifedata.com/resource/pubmed/id/18562705
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2008-9-26
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| pubmed:abstractText |
Phosphorylation of tyrosine residues in cellular proteins represents a major event during sperm capacitaton, but its relationship with the acquisition of sperm-fertilizing ability is still unclear. In this study we explored the relationship between the kinetics of the global tyrosine phosphorylation, monitored with a flow cytometric assay, and the acquisition of the human sperm ability to fuse with oocytes, evaluated with the progesterone-enhanced hamster egg penetration test. Sperm tyrosine phosphorylation appeared to be an early event in the capacitation process, with a 3.6-fold mean increase within 1 h of capacitation, but at this time sperm-oocyte fusion was extremely poor compared with that observed at 5 h of capacitation. Capacitation in calcium-free medium produced a 2-fold mean increase in tyrosine phosphorylation compared with that seen in complete capacitation medium both at 1 h and 5 h of capacitation, whereas sperm-oocyte fusion significantly increased only at 1 h, remaining unchanged at 5 h of capacitation. The cAMP analog, N,2-O-dibutyryladenosine 3',5'-cyclic monophosphate (dbcAMP), prevented the inhibitory effect of seminal plasma on tyrosine phosphorylation but not on sperm-oocyte fusion. In conclusion, these results suggest that the acquisition of sperm-fertilizing ability is always associated with an increase of the global tyrosine phosphorylation, but tyrosine phosphorylation does not necessarily reflect the acquisition of the sperm-fertilizing ability. Flow cytometry assay, a reliable technique to quickly quantify the global levels of the human sperm tyrosine phosphorylation, could be useful for a further elucidation of the biological meaning of this process, with the perspective of its clinical use as a measure of the sperm-fertilizing potential.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0006-3363
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
79
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
649-56
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:18562705-Animals,
pubmed-meshheading:18562705-Calcium,
pubmed-meshheading:18562705-Cricetinae,
pubmed-meshheading:18562705-Cyclic AMP,
pubmed-meshheading:18562705-Female,
pubmed-meshheading:18562705-Fertilization in Vitro,
pubmed-meshheading:18562705-Flow Cytometry,
pubmed-meshheading:18562705-Humans,
pubmed-meshheading:18562705-Kinetics,
pubmed-meshheading:18562705-Male,
pubmed-meshheading:18562705-Oocytes,
pubmed-meshheading:18562705-Phosphorylation,
pubmed-meshheading:18562705-Protein-Tyrosine Kinases,
pubmed-meshheading:18562705-Semen,
pubmed-meshheading:18562705-Sperm Capacitation,
pubmed-meshheading:18562705-Sperm-Ovum Interactions,
pubmed-meshheading:18562705-Spermatozoa,
pubmed-meshheading:18562705-Substrate Specificity
|
| pubmed:year |
2008
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| pubmed:articleTitle |
Dynamics of the global tyrosine phosphorylation during capacitation and acquisition of the ability to fuse with oocytes in human spermatozoa.
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| pubmed:affiliation |
Department of Internal Medicine, University of L'Aquila, 67100 L'Aquila, Italy.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
|