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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1991-8-23
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pubmed:abstractText |
Interleukin-8 (IL-8) is a newly described leukocyte chemotactic and activating cytokine that belongs to the novel family of inflammatory cytokines whose genes locate on human chromosome 4, q12-21 region. The production of IL-8 is usually not constitutive and can be induced rapidly and abundantly in different cell types by a variety of stimuli such as lipopolysaccharide, interleukin-1, tumor necrosis factor-alpha as well as a tumor promotor phorbol myristate acetate. We report here that in addition to these stimuli the IL-8 gene can also be induced by the protein X of the hepatitis B virus (HBV-X) as evidenced by the enhanced IL-8 mRNA expression and IL-8 production observed in HBV-X-transfected cells. Furthermore, using several deletion mutants of the 5'-flanking regulatory region of the human IL-8 gene linked to the chloramphenicol acetyl transferase gene as a reporter, we have established here that both nuclear factor kB and CCAAT/enhancer-binding protein-like cis-elements located at -94 to -71 base pairs of IL-8 gene are essential and sufficient for the induction of the IL-8 gene by HBV-X. The same elements have been identified recently by us to be interleukin-1-, tumor necrosis factor-alpha-, and phorbol myristate acetate-responsive elements on the IL-8 gene. This suggests the existence of a common pathway for these inflammatory cytokines and HBV-X to activate the IL-8 gene. These observations might be relevant to the pathogenesis of inflammation in viral hepatitis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/hepatitis B virus X protein
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
266
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13759-63
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:1856209-Base Sequence,
pubmed-meshheading:1856209-Blotting, Northern,
pubmed-meshheading:1856209-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:1856209-Cloning, Molecular,
pubmed-meshheading:1856209-DNA-Binding Proteins,
pubmed-meshheading:1856209-Gene Expression Regulation,
pubmed-meshheading:1856209-Hepatitis B Antigens,
pubmed-meshheading:1856209-Hepatitis B virus,
pubmed-meshheading:1856209-Humans,
pubmed-meshheading:1856209-Interleukin-1,
pubmed-meshheading:1856209-Interleukin-8,
pubmed-meshheading:1856209-Molecular Sequence Data,
pubmed-meshheading:1856209-NF-kappa B,
pubmed-meshheading:1856209-Nuclear Proteins,
pubmed-meshheading:1856209-Oligonucleotides,
pubmed-meshheading:1856209-RNA, Messenger,
pubmed-meshheading:1856209-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1856209-Tetradecanoylphorbol Acetate,
pubmed-meshheading:1856209-Trans-Activators,
pubmed-meshheading:1856209-Transcription, Genetic,
pubmed-meshheading:1856209-Transcription Factors,
pubmed-meshheading:1856209-Tumor Cells, Cultured,
pubmed-meshheading:1856209-Tumor Necrosis Factor-alpha
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pubmed:year |
1991
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pubmed:articleTitle |
Hepatitis B virus X protein transactivates human interleukin-8 gene through acting on nuclear factor kB and CCAAT/enhancer-binding protein-like cis-elements.
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pubmed:affiliation |
Department of Pharmacology, Kanazawa University, Ishikawa, Japan.
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pubmed:publicationType |
Journal Article
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