Source:http://linkedlifedata.com/resource/pubmed/id/18546847
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2008-6-12
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pubmed:abstractText |
Urine samples collected from 422 males and 53 females visiting a clinic in Kawasaki City who were suspected to have sexually transmitted infection were tested for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae by BD ProbeTecET (SDA method). The detection rates of C. trachomatis by the SDA method and polymerase chain reaction (PCR) method (control) were as high as 98.1% for C. trachomatis, and as high as 99.4% for N. gonorrhoeae, and the concordance rate of detection of both bacterial species was high. The detection sensitivity and specificity of the SDA method were 90.6 and 99.3%, respectively for C. trachomatis and 98.7% and 100% for N. gonorrhoeae, when PCR was used as the standard method. There were no differences in these results between males and females. The number of patients showing a discrepancy of the results obtained between the SDA method and the PCR method was 9 for C. trachomatis and 1 for N. gonorrhoeae, but the results of redetermination by the SDA method tended to coincide with those of the PCR method. Urine samples tested by the SDA method were positive for N. gonorrhoeae even in patients in whom the culture of secretions from the male urethra was negative for N. gonorrhoeae. Based on these results, the BD ProbeTecET (SDA method) was confirmed to have the equivalent capability to the PCR method for the detection of C. trachomatis and N. gonorrhoeae in urine samples.
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pubmed:language |
jpn
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0387-5911
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
182-6
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pubmed:dateRevised |
2008-12-16
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pubmed:meshHeading |
pubmed-meshheading:18546847-Chlamydia trachomatis,
pubmed-meshheading:18546847-Female,
pubmed-meshheading:18546847-Humans,
pubmed-meshheading:18546847-Male,
pubmed-meshheading:18546847-Neisseria gonorrhoeae,
pubmed-meshheading:18546847-Nucleic Acid Amplification Techniques,
pubmed-meshheading:18546847-Polymerase Chain Reaction,
pubmed-meshheading:18546847-Sensitivity and Specificity,
pubmed-meshheading:18546847-Sexually Transmitted Diseases, Bacterial,
pubmed-meshheading:18546847-Urine
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pubmed:year |
2008
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pubmed:articleTitle |
[Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine samples of males and females by the strand displacement amplification (SDA) method].
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pubmed:affiliation |
Infectious Disease Testing Department, Mitsubishi Chemical Medience Corporation.
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pubmed:publicationType |
Journal Article,
Comparative Study,
English Abstract,
Evaluation Studies
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