Linked Life Data

18546158

Source:http://linkedlifedata.com/resource/pubmed/id/18546158

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2008-7-17
pubmed:abstractText
Early embryonic losses are common in cloned embryos in the current cloning system. However, the reasons for embryonic losses in early developmental stages of cloned embryos remain unclear. To elucidate the cause of early defective development in cloned embryos, two porcine clones including extraembryonic tissues were obtained at 26 days of gestation. The expression of various molecules in developmentally important signaling pathways, including Notch, hedgehog (Hh), receptor tyrosine kinase (RTK), Janus kinase/signal transducer and activator of transcription (JAK/STAT), wingless related (Wnt), and transforming growth factor-beta (TGF-beta), was then examined in the extraembryonic tissues. Western blot analysis showed that the expression of key molecules involved in the Notch, Hh, RTK, and JAK/STAT signaling pathways was downregulated, whereas most Wnt and TGF-beta signaling pathway molecules were upregulated in cloned extraembryonic tissues compared to normal extraembryonic tissues. These results indicate that unbalanced coordination of signaling pathways might impair the early development of cloned embryos postimplantation, thereby resulting in embryonic losses during the first trimester.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1615-9861
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2724-34
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Aberrant expression of developmentally important signaling molecules in cloned porcine extraembryonic tissues.
pubmed:affiliation
Center for Regenerative Medicine, KRIBB, Daejeon, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't