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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
33
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pubmed:dateCreated |
2008-8-11
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pubmed:abstractText |
The alveolar surface of the lung is lined by alveolar type 1 (AT1) and type 2 (AT2) cells. Using single channel patch clamp analysis in lung slice preparations, we are able to uniquely study AT1 and AT2 cells separately from intact lung. We report for the first time the Na+ transport properties of type 2 cells accessed in live lung tissue (as we have done in type 1 cells). Type 2 cells in lung tissue slices express both highly selective cation and nonselective cation channels with average conductances of 8.8 +/- 3.2 and 22.5 +/- 6.3 picosiemens, respectively. Anion channels with 10-picosiemen conductance are also present in the apical membrane of type 2 cells. Our lung slice studies importantly verify the use of cultured cell model systems commonly used in lung epithelial sodium channel (ENaC) studies. Furthermore, we identify novel functional differences between the cells that make up the alveolar epithelium. One important difference is that exposure to the nitric oxide (NO) donor, PAPA-NONOate (1.5 microm), significantly decreases average ENaC NPo in type 2 cells (from 1.38 +/- 0.26 to 0.82 +/- 0.16; p < 0.05 and n = 18) but failed to alter ENaC activity in alveolar type 1 cells. Elevating endogenous superoxide (O2.) levels with Ethiolat, a superoxide dismutase inhibitor, prevented NO inhibition of ENaC activity in type 2 cells, supporting the novel hypothesis that O2. and NO signaling plays an important role in maintaining lung fluid balance.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-10744680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-11283228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-11686863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-12381774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-12426202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-15150284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-1540393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-15463939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-16497717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-17158884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-17296874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-2806398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-3382696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-3637065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-7742013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-8398161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-8589728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-9376126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-9465058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-9575865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541535-9755109
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
283
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
22875-83
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:18541535-Animals,
pubmed-meshheading:18541535-Cell Membrane,
pubmed-meshheading:18541535-Epithelial Sodium Channel,
pubmed-meshheading:18541535-Lung,
pubmed-meshheading:18541535-Male,
pubmed-meshheading:18541535-Microscopy, Fluorescence,
pubmed-meshheading:18541535-Oxidation-Reduction,
pubmed-meshheading:18541535-Oxygen Consumption,
pubmed-meshheading:18541535-Patch-Clamp Techniques,
pubmed-meshheading:18541535-Pulmonary Alveoli,
pubmed-meshheading:18541535-Rats,
pubmed-meshheading:18541535-Rats, Sprague-Dawley
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pubmed:year |
2008
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pubmed:articleTitle |
Redox regulation of epithelial sodium channels examined in alveolar type 1 and 2 cells patch-clamped in lung slice tissue.
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pubmed:affiliation |
Department of Physiology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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