Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1991-5-21
pubmed:abstractText
Treatment of A431 human epidermoid cells with epidermal growth factor (EGF; 20 nM) results in decreased proliferation. This is associated with blockage of the cells in the S and/or G2 phases of the cell cycle. We found that tyrphostin, a putative tyrosine kinase inhibitor, in the range of 50 to 100 microM, partially reversed the growth-inhibitory and cell cycle changes induced by EGF. By using high-pressure liquid chromatography with electrochemical detection, we found that tyrphostin was readily incorporated into A431 cells, reaching maximal levels within 1 h. Although tyrphostin (50 to 100 microM) had no effect on high-affinity binding of EGF to its receptor in A431 cells for up to 24 h, the compound partially inhibited EGF-stimulated EGF receptor tyrosine kinase activity. However, this effect was evident only after prolonged treatment of the cells (4 to 24 h) with the drug. When the peak intracellular concentration of tyrphostin occurred (1 h), no inhibition of tyrosine kinase activity was observed. After both 1 and 24 h, tyrphostin was a less effective inhibitor of tyrosine kinase activity than the potent tumor promoter 12-O-tetradecanoyl phorbol-13-acetate, which almost completely blocked EGF receptor autophosphorylation. On the basis of our data, we hypothesize that tyrphostin is not a competitive inhibitor of the EGF receptor tyrosine kinase in intact cells and that it functions by an indirect mechanism.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-2472218, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-2492663, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-2552117, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-2555218, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-2557535, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-2682241, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-2784435, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-2788167, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-2887282, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-2984676, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-3021781, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-3039909, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-308698, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-314449, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-3263702, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-3476196, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-3490664, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-3584259, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-4128323, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-6090944, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-6090945, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-6094567, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-6196603, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-6232463, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-6268987, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-6276390, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-6283535, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-6296169, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-6305956, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-6321473, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-6328489, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-7040412, http://linkedlifedata.com/resource/pubmed/commentcorrection/1850101-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2697-703
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Rapid uptake of tyrphostin into A431 human epidermoid cells is followed by delayed inhibition of epidermal growth factor (EGF)-stimulated EGF receptor tyrosine kinase activity.
pubmed:affiliation
Department of Environmental and Community Medicine, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.