Linked Life Data

1843343

Source:http://linkedlifedata.com/resource/pubmed/id/1843343

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1993-2-25
pubmed:abstractText
Embryos of amphibians, sheep, cattle, pigs and rabbits have been multiplied by nuclear transfer. Successful nuclear transfer in these species has been accomplished by transfer of a blastomere from a late-stage embryo into an enucleated oocyte with large scale multiplication by repeating the procedure using blastomeres from the embryos produced from nuclear transfer. This allows the production of clonal lines which, when appropriately selected for performance in a given trait, can be reproduced to capture in the offspring expression of additive and non-additive inheritance. The efficiency of these procedures is high only for amphibian embryos for which as many as 1000 offspring can be made from a blastula- or gastrula-stage embryo and the process repeated 60-100 times with descendant embryos. In domestic animals the largest number of offspring from one embryo has been 8 calves. Embryos as late as the 64-cell stage in cattle and 120-cell blastocyst in sheep have been used successfully as donors of blastomeres. Recloning has also been done in cattle. Nuclear transfer potentially provides a mechanism for multiplication and production testing of clonal lines, a method for rapid genetic improvement and rapid propagation of a selected genotype. Unfortunately the present efficiencies of subsequent embryo development, pregnancy and embryo survival are less than normal. This paper reviews variables contributing to reduced efficiency and research to improve nuclear transfer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0449-3087
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
245-54
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Production of embryos by oocyte cytoplast-blastomere fusion in domestic animals.
pubmed:affiliation
Department of Meat and Animal Science, University of Wisconsin, Madison 53706.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't