Linked Life Data

18388994

Source:http://linkedlifedata.com/resource/pubmed/id/18388994

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-4-4
pubmed:abstractText
We described 2 unusual Neisseria meningitidis strains isolated from epidemiologically unrelated invasive meningococcal disease cases in Ontario, Canada. Both isolates have features typical of serogroup Y N. meningitidis: are of serotype 2c, are of the multi-locus sequence types typical of the serogroup Y strains in Canada, and are genotyped as serogroup Y based on a previously described PCR-ELISA method that detects the serogroup-Y-specific siaD gene. However, both strains were poly-agglutinable in both anti-Y and anti-W135 antisera. Further studies on 1 of these 2 isolates showed the presence of glucose and galactose as well as sialic acids in its purified capsular polysaccharide, suggesting the presence of both serogroup Y and serogroup W135 polysaccharides. Rabbit antisera produced to this strain contained antibodies to both purified serogroup Y and serogroup W135 capsular polysaccharides. Absorption experiments with either serogroup Y or serogroup W135 bacteria confirmed the presence of antibodies to these 2 different polysaccharides. DNA sequencing of the cps operon from both isolates revealed a siaD gene with 99.7% homology to the published siaD sequence from a serogroup Y strain but with 3 point mutations that all resulted in amino acid changes. How these strains may affect results of routine surveillance, PCR diagnosis, and immuno-protection by vaccination are discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0008-4166
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
229-34
pubmed:meshHeading
pubmed-meshheading:18388994-Amino Acid Substitution, pubmed-meshheading:18388994-Antibodies, Bacterial, pubmed-meshheading:18388994-Bacterial Capsules, pubmed-meshheading:18388994-Bacterial Proteins, pubmed-meshheading:18388994-Base Sequence, pubmed-meshheading:18388994-Child, Preschool, pubmed-meshheading:18388994-DNA, Bacterial, pubmed-meshheading:18388994-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:18388994-Female, pubmed-meshheading:18388994-Galactose, pubmed-meshheading:18388994-Genotype, pubmed-meshheading:18388994-Glucose, pubmed-meshheading:18388994-Humans, pubmed-meshheading:18388994-Infant, Newborn, pubmed-meshheading:18388994-Male, pubmed-meshheading:18388994-Meningococcal Infections, pubmed-meshheading:18388994-Molecular Sequence Data, pubmed-meshheading:18388994-Neisseria meningitidis, pubmed-meshheading:18388994-Ontario, pubmed-meshheading:18388994-Point Mutation, pubmed-meshheading:18388994-Polymerase Chain Reaction, pubmed-meshheading:18388994-Sequence Analysis, DNA, pubmed-meshheading:18388994-Serotyping, pubmed-meshheading:18388994-Sialic Acids
pubmed:year
2008
pubmed:articleTitle
Immunochemical studies and genetic background of two Neisseria meningitidis isolates expressing unusual capsule polysaccharide antigens with specificities of both serogroup Y and W135.
pubmed:affiliation
Laboratory for Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E3R2, Canada. raymond_tsang@phac-aspc.gc.ca
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't