Linked Life Data

18339999

Source:http://linkedlifedata.com/resource/pubmed/id/18339999

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-3-14
pubmed:databankReference
pubmed:abstractText
Divalent metal transporter 1 (DMT1) is an electrogenic transporter of divalent Mn that is expressed at a high level on the brush-border membrane of enterocytes. This study is the first reported isolation of the 2 full-length cDNA sequences of the small intestinal DMT1 gene of broilers by rapid amplification of cDNA ends. The chicken DMT1 isoform I cDNA was 1,972 bp and contained a 1,695-bp open reading frame encoding a 564-amino acid protein, and the chicken DMT1 isoform II cDNA was 1,775 bp and contained a 1,593-bp open reading frame encoding a 530-amino acid protein. The 2 chicken DMT1 isoform transcripts differed in their 3'-translated regions and untranslated regions. The identities of the amino acid sequence deduced from the full-length cDNA sequence of the chicken DMT1 isoform I with DMT1 not containing the iron-responsive element of the mouse, rat, and human were 82, 82, and 80%, respectively. The identities of the amino acid sequence deduced from the full-length cDNA sequence of the chicken DMT1 isoform II with those of the mouse, rat, and human were 84, 84, and 83%, respectively. Analyses of hydrophobicity, transmembrane region, and signal peptides of DMT1 proteins deduced by nucleotide sequences suggested that chicken DMT1 isoforms are transmembrane proteins with several conserved peptide sequences, such as N-linked glycosylation signals (N-X-S/ T, where X designates any amino acid) and the consensus transport motif. The total mRNA levels of the 2 chicken DMT1 isoforms, the mRNA levels of chicken DMT1 isoform I, and the mRNA levels of chicken DMT1 isoform II in the duodenum and jejunum were higher (P<0.002) than that in the ileum by real-time reverse transcription PCR assay. There was no significant difference (P>0.26) between the duodenum and jejunum for the above 3 indices. The mRNA level of the chicken DMT1 isoform I was higher (P<0.001) than that of the chicken DMT1 isoform II in each small intestinal segment of Mn-deficient broilers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0032-5791
pubmed:author
pubmed:issnType
Print
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
768-76
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Cloning, sequencing, characterization, and expressions of divalent metal transporter one in the small intestine of broilers.
pubmed:affiliation
Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't