Source:http://linkedlifedata.com/resource/pubmed/id/18327798
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2008-7-21
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| pubmed:abstractText |
We evaluated whether proinflammatory cytokine expression and myofibroblast recruitment in subacromial bursa was linked to rotator cuff lesions with shoulder stiffness. We analyzed expressions of IL-1beta, IL-6, and TNF-alpha in subacromial bursa and joint fluid collected from 14 patients with cuff tears with stiffness as a study group (Group I) and 14 patients with rotator cuff tears without shoulder stiffness as a control group (Group II) using real-time RT-PCR, immunohistochemistry, and ELISA. Myofibroblast apoptosis in subacromial bursa was analyzed using terminal deoxynucleotidyl transferase -mediated deoxyuridine triphosphate-biotin nick end-labeling (TUNEL) and alpha-smooth muscle actin immunofluorescence staining. Shoulder function was evaluated using the Constant score. Group I had higher mRNA expression (p < 0.001) and immunoreactivities (p < 0.001) of IL-1beta. They also had higher levels of IL-1beta, IL-6, and TNF-alpha in joint fluid. Increased IL-1beta mRNA expression in the subacromial bursa and IL-1beta levels in joint fluid were correlated with a preoperative deficit in shoulder motion (p < 0.001) and preoperative Constant scores (p < 0.001). Immunofluorescence observations showed that Group I subjects had more myofibroblasts (p < 0.001) than Group II. In Group II, a significant correlation was found between apoptotic myofibroblasts and total myofibroblasts (p = 0.002), but not in Group I (p = 0.510). Increased expression of IL-1beta and myofibroblast recruitment in the subacromial bursa in rotator cuff lesions are linked to shoulder stiffness.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
1554-527X
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
26
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1090-7
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| pubmed:meshHeading |
pubmed-meshheading:18327798-Acromioclavicular Joint,
pubmed-meshheading:18327798-Aged,
pubmed-meshheading:18327798-Apoptosis,
pubmed-meshheading:18327798-Bursa, Synovial,
pubmed-meshheading:18327798-Female,
pubmed-meshheading:18327798-Fibroblasts,
pubmed-meshheading:18327798-Fluorescent Antibody Technique,
pubmed-meshheading:18327798-Gene Expression,
pubmed-meshheading:18327798-Humans,
pubmed-meshheading:18327798-In Situ Nick-End Labeling,
pubmed-meshheading:18327798-Interleukin-1beta,
pubmed-meshheading:18327798-Interleukin-6,
pubmed-meshheading:18327798-Male,
pubmed-meshheading:18327798-Middle Aged,
pubmed-meshheading:18327798-RNA, Messenger,
pubmed-meshheading:18327798-Rotator Cuff,
pubmed-meshheading:18327798-Synovial Fluid,
pubmed-meshheading:18327798-Tumor Necrosis Factor-alpha
|
| pubmed:year |
2008
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| pubmed:articleTitle |
Increased IL-1beta expression and myofibroblast recruitment in subacromial bursa is associated with rotator cuff lesions with shoulder stiffness.
|
| pubmed:affiliation |
Department of Orthopaedic Surgery, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Taiwan, Republic of China. kojy@adm.cgmh.org.tw
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|