Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2008-5-29
pubmed:abstractText
The TATA-binding protein (TBP) is a key component of the archaea ternary preinitiation transcription assembly. The archaeon TBP, from the halophile/hyperthermophile organism Pyrococcus woesei, is adapted to high concentrations of salt and high-temperature environments. Although most eukaryotic TBPs are mesophilic and adapted to physiological conditions of temperature and salt, they are very similar to their halophilic counterparts in sequence and fold. However, whereas the binding affinity to DNA of halophilic TBPs increases with increasing salt concentration, the opposite is observed for mesophilic TBPs. We investigated these differences in nonspecific salt-dependent DNA-binding behavior of halophilic and mesophilic TBPs by using a combined molecular mechanics/Poisson-Boltzmann approach. Our results are qualitatively in good agreement with experimentally observed salt-dependent DNA-binding for mesophilic and halophilic TBPs, and suggest that the distribution and the total number of charged residues may be the main underlying contributor in the association process. Therefore, the difference in the salt-dependent binding behavior of mesophilic and halophilic TBPs to DNA may be due to the very unique charge and electrostatic potential distribution of these TBPs, which consequently alters the number of repulsive and attractive electrostatic interactions.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-1003464, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-10222198, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-10570130, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-10617571, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-11327862, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-11428896, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-11551792, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-11959501, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-12015146, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-14695279, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-14725775, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-15027106, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-17238171, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-17378582, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-17488841, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-2410050, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-3227015, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-353876, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-6548264, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-6887253, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-8413604, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-8610010, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-8643494, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-8696975, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-8757291, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-9000631, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-9177165, http://linkedlifedata.com/resource/pubmed/commentcorrection/18326635-9636704
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1542-0086
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4634-45
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Salt-mediated electrostatics in the association of TATA binding proteins to DNA: a combined molecular mechanics/Poisson-Boltzmann study.
pubmed:affiliation
Institute of Molecular Biophysics and Department of Physics, Florida State University, Tallahassee, Florida 32306, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.