Linked Life Data

18314732

Source:http://linkedlifedata.com/resource/pubmed/id/18314732

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-3-4
pubmed:abstractText
The preparation of mammalian mitochondrial ribosomes in sufficient quantities for biochemical studies is best done beginning with whole tissue rather than from cells in culture. This issue arises because of the low abundance of these ribosomes in cells, making their isolation a challenge. Crude mitochondrial preparations are made by differential centrifugation and are treated with digitonin to remove the outer membrane. This treatment also effectively removes most contamination by cytoplasmic ribosomes. Purification of mammalian mitochondrial ribosomes requires treatment with detergents to release the ribosomes from their association with the membrane. Sucrose density gradient centrifugation is used to separate the ribosomes from other large oligomeric complexes from this organelle.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
372
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
265-75
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Large-scale isolation of mitochondrial ribosomes from mammalian tissues.
pubmed:affiliation
Department of Chemistry, University of North Carolina, Chapel Hill, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural