Source:http://linkedlifedata.com/resource/pubmed/id/18289674
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rdf:type | |
lifeskim:mentions |
umls-concept:C0005919,
umls-concept:C0022742,
umls-concept:C0033268,
umls-concept:C0038585,
umls-concept:C0039099,
umls-concept:C0061132,
umls-concept:C0086418,
umls-concept:C0164209,
umls-concept:C0205276,
umls-concept:C0302523,
umls-concept:C0700325,
umls-concept:C1280500,
umls-concept:C1706089,
umls-concept:C2745955
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pubmed:issue |
2
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pubmed:dateCreated |
2008-3-7
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pubmed:abstractText |
We have previously shown that levels of the neuropeptides substance P (SP) and bombesin/gastrin-releasing peptide (BN/GRP) in blood and synovial fluid correlate with levels of pro-inflammatory cytokines in patients with rheumatoid arthritis (RA). It is well-established that SP is present in nerve endings in the synovium whilst the source of BN/GRP in human joints is completely unknown. Nor is it known whether GRP-receptors (GRP-R) are present in human synovial tissue. This study aimed to investigate the expression pattern of SP, BN/GRP and their receptors (NK-1R and GRP-R) in synovial tissue. Synovial tissue specimens from patients with RA or osteoarthritis (OA) were processed for immunohistochemistry, in situ hybridisation and ELISA. The results show the presence of BN/GRP, but not SP, in cells in the synovial tissue at both the protein and mRNA level. We did not find immunoreactive BN/GRP in nerve structures. NK-1R and GRP-R were also expressed at both protein and mRNA levels in cells associated with blood vessels and cells in the interstitial tissue. ELISA analyses revealed both SP and BN/GRP to be present in synovial tissue extracts and that synovial levels of SP were higher in RA patients than those with OA. Our results indicate that BN/GRP is produced by non-neuronal cells in the synovial tissue. Furthermore, both BN/GRP and SP may exert their effects on the synovial tissue through the respective receptors. These results suggest that BN/GRP and SP may modulate inflammation and vascular events, and possibly healing processes in the synovium. Finally, nerves should not be considered as the source of BN/GRP in synovial tissue although this peptide is presumably intimately involved functionally in synovial tissue, a previously unrecognised fact.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bombesin,
http://linkedlifedata.com/resource/pubmed/chemical/Gastrin-Releasing Peptide,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Neurokinin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Substance P
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0143-4179
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
133-45
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pubmed:meshHeading |
pubmed-meshheading:18289674-Adult,
pubmed-meshheading:18289674-Aged,
pubmed-meshheading:18289674-Arthritis, Rheumatoid,
pubmed-meshheading:18289674-Bombesin,
pubmed-meshheading:18289674-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:18289674-Gastrin-Releasing Peptide,
pubmed-meshheading:18289674-Humans,
pubmed-meshheading:18289674-Immunohistochemistry,
pubmed-meshheading:18289674-In Situ Hybridization,
pubmed-meshheading:18289674-Knee Joint,
pubmed-meshheading:18289674-Middle Aged,
pubmed-meshheading:18289674-Osteoarthritis, Knee,
pubmed-meshheading:18289674-RNA, Messenger,
pubmed-meshheading:18289674-Receptors, Neurokinin-1,
pubmed-meshheading:18289674-Staining and Labeling,
pubmed-meshheading:18289674-Substance P,
pubmed-meshheading:18289674-Synovial Membrane
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pubmed:year |
2008
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pubmed:articleTitle |
Observations favouring the occurrence of local production and marked effects of bombesin/gastrin-releasing peptide in the synovial tissue of the human knee joint--comparisons with substance P and the NK-1 receptor.
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pubmed:affiliation |
Section for Anatomy, Department of Integrative Medical Biology, Umeå University, and Department of Rheumatology, Umeå University Hospital, SE-901 87 Umeå, Sweden. ola.grimsholm@anatomy.umu.se
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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