Linked Life Data

18228427

Source:http://linkedlifedata.com/resource/pubmed/id/18228427

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-1-29
pubmed:abstractText
This unit presents two methods of calcium phosphate-based eukaryotic cell transfection that can be used for both transient and stable transfections. In these protocols, plasmid DNA is introduced to monolayer cell cultures via a precipitate that adheres to the cell surface. A HEPES-buffered solution is used to form a calcium phosphate precipitate that is directly layered onto the cells. For some cells, shocking the cells with glycerol or DMSO improves transfection efficiency. In the alternate high-efficiency method, a BES-buffered system is used that allows the precipitate to form gradually in the medium and then drop onto the cells. While the alternate method is particularly efficient for stable transformation of cells with circular plasmid DNA, both protocols yield similar results for transformation with linear plasmid or genomic DNA, or for transient expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1934-2616
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 20.3
pubmed:dateRevised
2009-12-11
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Calcium phosphate transfection.
pubmed:affiliation
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
pubmed:publicationType
Journal Article