|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0038250,
umls-concept:C0086418,
umls-concept:C0162371,
umls-concept:C0205245,
umls-concept:C0225828,
umls-concept:C0282547,
umls-concept:C0871161,
umls-concept:C1301820,
umls-concept:C1332710
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2008-4-2
|
|
pubmed:abstractText |
Prior in vitro studies suggested that different types of hematopoietic stem cells may differentiate into cardiomyocytes. The present work examined whether human CD34(+) cells from the human umbilical cord blood (hUCB), cocultured with neonatal mouse cardiomyocytes, acquire the functional properties of myocardial cells and express human cardiac genes. hUCB CD34(+) cells were cocultured onto cardiomyocytes following an infection with a lentivirus-encoding enhanced green fluorescent protein (EGFP). After 7 days, mononucleated EGFP(+) cells were tested for their electrophysiological features by patch clamp and for cytosolic [Ca(2+)] ([Ca(2+)](i)) homeostasis by [Ca(2+)](i) imaging of X-rhod1-loaded cells. Human Nkx2.5 and GATA-4 expression was examined in cocultured cell populations by real-time RT-PCR. EGFP(+) cells were connected to surrounding cells by gap junctions, acquired electrophysiological properties similar to those of cardiomyocytes, and showed action potential-associated [Ca(2+)](i) transients. These cells also exhibited spontaneous sarcoplasmic reticulum [Ca(2+)](i) oscillations and the associated membrane potential depolarization. However, RT-PCR of both cell populations showed no upregulation of human-specific cardiac genes. In conclusion, under our experimental conditions, hUCB CD34(+) cells cocultured with murine cardiomyocytes formed cells that exhibited excitation-contraction coupling features similar to those of cardiomyocytes. However, the expression of human-specific cardiac genes was undetectable by RT-PCR.
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/GATA4 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GATA4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NKX2-5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/enhanced green fluorescent protein
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0363-6135
|
|
pubmed:author |
pubmed-author:AvitabileDanieleD,
pubmed-author:BonannoGiuseppinaG,
pubmed-author:CapogrossiMaurizio CMC,
pubmed-author:EusebiFabrizioF,
pubmed-author:FucileSergioS,
pubmed-author:GrassiFrancescaF,
pubmed-author:OrlandiAlessiaA,
pubmed-author:PaganiFrancescaF,
pubmed-author:PesceMaurizioM,
pubmed-author:ScambiaGiovanniG,
pubmed-author:VignaElisaE
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
294
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
H1541-9
|
|
pubmed:meshHeading |
pubmed-meshheading:18223188-Animals,
pubmed-meshheading:18223188-Animals, Newborn,
pubmed-meshheading:18223188-Antigens, CD34,
pubmed-meshheading:18223188-Calcium,
pubmed-meshheading:18223188-Cell Communication,
pubmed-meshheading:18223188-Cell Differentiation,
pubmed-meshheading:18223188-Cell Shape,
pubmed-meshheading:18223188-Cell Transdifferentiation,
pubmed-meshheading:18223188-Cells, Cultured,
pubmed-meshheading:18223188-Coculture Techniques,
pubmed-meshheading:18223188-Fetal Blood,
pubmed-meshheading:18223188-GATA4 Transcription Factor,
pubmed-meshheading:18223188-Gap Junctions,
pubmed-meshheading:18223188-Genes, Reporter,
pubmed-meshheading:18223188-Genetic Vectors,
pubmed-meshheading:18223188-Green Fluorescent Proteins,
pubmed-meshheading:18223188-Homeodomain Proteins,
pubmed-meshheading:18223188-Humans,
pubmed-meshheading:18223188-Lentivirus,
pubmed-meshheading:18223188-Membrane Potentials,
pubmed-meshheading:18223188-Mice,
pubmed-meshheading:18223188-Myocytes, Cardiac,
pubmed-meshheading:18223188-Patch-Clamp Techniques,
pubmed-meshheading:18223188-RNA, Messenger,
pubmed-meshheading:18223188-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18223188-Sarcoplasmic Reticulum,
pubmed-meshheading:18223188-Stem Cells,
pubmed-meshheading:18223188-Time Factors,
pubmed-meshheading:18223188-Transcription Factors,
pubmed-meshheading:18223188-Transduction, Genetic
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Functional properties of cells obtained from human cord blood CD34+ stem cells and mouse cardiac myocytes in coculture.
|
|
pubmed:affiliation |
Laboratorio di Biologia Vascolare e Terapia Genica, Centro Cardiologico Monzino IRCCS, Via Parea 4, 20138 Milan, Italy.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
